Application
Fig1: Western blot analysis of Cyclin B2 on different lysates using anti-Cyclin B2 antibody at 1/1,000 dilution. Positive control: Lane 1: Hela Lane 2: K562Application
Fig2: ICC staining Cyclin B2 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig3: ICC staining Cyclin B2 in PC-12 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig4: Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-Cyclin B2 antibody. Counter stained with hematoxylin.Application
Fig5: Immunohistochemical analysis of paraffin-embedded mouse colon tissue using anti-Cyclin B2 antibody. Counter stained with hematoxylin.| Product Name | Cyclin B2 [SD2045] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | ccnb2 antibody CCNB2_HUMAN antibody CycB2 antibody Cyclin B2 antibody G2 mitotic specific cyclin B2 antibody G2/mitotic specific cyclin B2 antibody G2/mitotic-specific cyclin-B2 antibody HsT17299 antibody MGC108931 antibody MGC140694 antibody |
| Product description | In eukaryotic cells, mitosis is initiated following the activation of a protein kinase known variously as maturation-promoting factor, M-phase specific histone kinase or M-phase kinase. This protein kinase is composed of a catalytic subunit (Cdc2), a regulatory subunit (cyclin B) and a low molecular weight subunit (p13-Suc 1). The Cdc/cyclin enzyme is subject to multiple levels of control of which the regulation of the catalytic subunit by tyrosine phosphorylation is the best understood. Tyrosine phosphorylation inhibits the Cdc2/cyclin B enzyme and tyrosine dephosphorylation, occurring at the onset of mitosis, directly activates the pre-MPF complex . Evidence has established that B-type cyclins not only act on M-phase regulatory subunits of the Cdc2 protein kinase, but also activate the Cdc25A and Cdc25B endogenous tyrosine phosphatase, of which Cdc2 is the physiological substrate. The two B-type cyclins, cyclin B1 and cyclin B2, have been shown to have distinct tissue distributions. |
| Immunogen | recombinant protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Recombinant rabbit |
| Tested Applications | WBICC/IFIHCIP |
| WB:1:1,000 ICC:1:50-1:200 IHC:1:50-1:200 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Alternative Names | ccnb2 antibody CCNB2_HUMAN antibody CycB2 antibody Cyclin B2 antibody G2 mitotic specific cyclin B2 antibody G2/mitotic specific cyclin B2 antibody G2/mitotic-specific cyclin-B2 antibody HsT17299 antibody MGC108931 antibody MGC140694 antibody |
|---|---|
| Molecular Weight(MW) | 45 kDa |
| Cellular Localization | Cytoplasm, Nucleus |
Application
Fig1: Western blot analysis of Cyclin B2 on different lysates using anti-Cyclin B2 antibody at 1/1,000 dilution. Positive control: Lane 1: Hela Lane 2: K562
Application
Fig2: ICC staining Cyclin B2 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig3: ICC staining Cyclin B2 in PC-12 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig4: Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-Cyclin B2 antibody. Counter stained with hematoxylin.
Application
Fig5: Immunohistochemical analysis of paraffin-embedded mouse colon tissue using anti-Cyclin B2 antibody. Counter stained with hematoxylin.| Positive Control | K562, PC-12, Hela, mouse testis tissue, mouse colon tissue. |
|---|---|
| Application Notes | WB:1:1,000 ICC:1:50-1:200 IHC:1:50-1:200 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
