Application
Fig1: Western blot analysis of Cdk6 on different lysates using anti-Cdk6 antibody at 1/1,000 dilution. Positive control: Lane 1: Jurkat Lane 2: K562 Lane 3: HelaApplication
Fig2: ICC staining Cdk6 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig3: ICC staining Cdk6 in SW480 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig4: ICC staining Cdk6 in 293 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig5: Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-Cdk6 antibody. Counter stained with hematoxylin.Application
Fig6: Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-Cdk6 antibody. Counter stained with hematoxylin.Application
Fig7: Flow cytometric analysis of Hela cells with Cdk6 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.| Product Name | Cdk6 [SD20-50] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | CDK 6 antibody CDK6 antibody CDK6_HUMAN antibody Cell division protein kinase 6 antibody Crk 2 antibody Crk2 antibody Cyclin dependent kinase 6 antibody Cyclin-dependent kinase 6 antibody MGC59692 antibody p40 antibody PLSTIRE antibody Serine/threonine protein kinase PLSTIRE antibody Serine/threonine-protein kinase PLSTIRE antibody STQTL11 antibody |
| Product description | Cell cycle progression is controlled in part by a family of cyclin proteins and cyclin dependent kinases (Cdks). Cdk proteins work in concert with the cyclins to phosphorylate key substrates involved in each phase of cell cycle progression. Another family of proteins, Cdk inhibitors, also plays a role in regulating the cell cycle by binding to cyclin-Cdk complexes and modulating their activity. Several Cdk proteins have been identified, including Cdk2-Cdk8, PCTAIRE-1-PCTAIRE-3, PITALRE and PITSLRE. Cdk6 is known to associate with cyclins D1, D2 and D3 and to be involved with the G1/S transition of the cell cycle. Multiple inhibitors of Cdk6 have been identified, including p18 and p19. These inhibitors bind to both free and complexed Cdk6, and they inhibit the activity of the cyclin D-bound Cdk6. |
| Immunogen | recombinant protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Recombinant rabbit |
| Tested Applications | WBICC/IFIHCFC |
| WB:1:1,000-1:2,000 ICC:1:50-1:200 IHC:1:50-1:200 FC:1:50-1:100 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Alternative Names | CDK 6 antibody CDK6 antibody CDK6_HUMAN antibody Cell division protein kinase 6 antibody Crk 2 antibody Crk2 antibody Cyclin dependent kinase 6 antibody Cyclin-dependent kinase 6 antibody MGC59692 antibody p40 antibody PLSTIRE antibody Serine/threonine protein kinase PLSTIRE antibody Serine/threonine-protein kinase PLSTIRE antibody STQTL11 antibody |
|---|---|
| Molecular Weight(MW) | 37 kDa |
| Cellular Localization | Cytoplasm, Nucleus, Cell projection. |
| SwissProt ID | Q00534 |
|---|
Application
Fig1: Western blot analysis of Cdk6 on different lysates using anti-Cdk6 antibody at 1/1,000 dilution. Positive control: Lane 1: Jurkat Lane 2: K562 Lane 3: Hela
Application
Fig2: ICC staining Cdk6 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig3: ICC staining Cdk6 in SW480 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig4: ICC staining Cdk6 in 293 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig5: Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-Cdk6 antibody. Counter stained with hematoxylin.
Application
Fig6: Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-Cdk6 antibody. Counter stained with hematoxylin.
Application
Fig7: Flow cytometric analysis of Hela cells with Cdk6 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.| Positive Control | SW480, 293, Hela, Jurkat, K562, human tonsil tissue, human colon cancer tissue. |
|---|---|
| Application Notes | WB:1:1,000-1:2,000 ICC:1:50-1:200 IHC:1:50-1:200 FC:1:50-1:100 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
