Application
Fig1: ICC staining Phospho-Cyclin E1(T77) in SW480 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig2: ICC staining Phospho-Cyclin E1(T77) in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig3: ICC staining Phospho-Cyclin E1(T77) in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig4: Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-Phospho-Cyclin E1(T77) antibody. Counter stained with hematoxylin.Application
Fig5: Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-Phospho-Cyclin E1(T77) antibody. Counter stained with hematoxylin.| Product Name | Phospho-Cyclin E1(T77) [SD2025] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | CCNE antibody Ccne1 antibody CCNE1_HUMAN antibody cyclin E variant ex5del antibody cyclin E variant ex7del antibody Cyclin E1 antibody Cyclin Es antibody Cyclin Et antibody CyclinE antibody G1/S specific cyclin E antibody G1/S-specific cyclin-E1 antibody |
| Product description | Cyclins were first identified in invertebrates as proteins that oscillate dramatically through the cell cycle. These proteins have been well conserved through evolution and play a critical role in regulation of cell division. cyclin E, along with the three cyclin D proteins and cyclin C, has been shown to represent a putative G1 cyclin on the basis of its cyclic pattern of mRNA expression, with maximal levels being detected near the G1/S boundary. cyclin E has been found to be associated with the transcription factor E2F in a temporally regulated manner. The cyclin E/E2F complex is detected primarily during the G1 phase of the cell cycle and decreases as cells enter S phase. E2F is known to be a critical transcription factor for expression of several S phase specific proteins. |
| Immunogen | Synthetic phospho-peptide corresponding to residues surrounding Thr77 of human Cyclin E1. |
| Modification | p-T77 |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Recombinant rabbit |
| Tested Applications | WBICC/IFIHC |
| WB:1:1,000 ICC:1:50-1:200 IHC:1:50-1:200 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Alternative Names | CCNE antibody Ccne1 antibody CCNE1_HUMAN antibody cyclin E variant ex5del antibody cyclin E variant ex7del antibody Cyclin E1 antibody Cyclin Es antibody Cyclin Et antibody CyclinE antibody G1/S specific cyclin E antibody G1/S-specific cyclin-E1 antibody |
|---|---|
| Molecular Weight(MW) | 48 kDa |
| Cellular Localization | Nucleus. |
| SwissProt ID | P24864 |
|---|
Application
Fig1: ICC staining Phospho-Cyclin E1(T77) in SW480 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig2: ICC staining Phospho-Cyclin E1(T77) in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig3: ICC staining Phospho-Cyclin E1(T77) in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig4: Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-Phospho-Cyclin E1(T77) antibody. Counter stained with hematoxylin.
Application
Fig5: Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-Phospho-Cyclin E1(T77) antibody. Counter stained with hematoxylin.| Positive Control | SW480, Hela, HepG2, human tonsil tissue, human colon cancer tissue. |
|---|---|
| Application Notes | WB:1:1,000 ICC:1:50-1:200 IHC:1:50-1:200 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
