Application
Fig1: Western blot analysis of MEK5 on Hela cells lysates using anti-MEK5 antibody at 1/1,000 dilution.Application
Fig2: ICC staining MEK5 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig3: ICC staining MEK5 in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig4: ICC staining MEK5 in SW480 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.| Product Name | MEK5 [SD208-6] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Dual specificity mitogen activated protein kinase kinase 5 antibody Dual specificity mitogen-activated protein kinase kinase 5 antibody EC 2.7.12.2 antibody HsT17454 antibody MAP kinase kinase 5 antibody MAP kinase kinase MEK5b antibody MAP2K5 antibody MAPK/ERK kinase 5 antibody MAPKK 5 antibody MAPKK5 antibody MEK 5 antibody mitogen-activated protein kinase kinase 5 antibody MKK5 antibody MP2K5_HUMAN antibody PRKMK5 antibody Protein kinase, mitogen-activated, kinase 5 antibody SAPKK5 antibody SKK5 antibody |
| Product description | A family of protein kinases located upstream of the MAP kinases and responsible for their activation has been identified. The prototype member of this family, designated MAP kinase kinase, or MEK-1, specifically phosphorylates the MAP kinase regulatory threonine and tyrosine residues present in the Thr-Glu-Tyr motif of ERK. A second MEK family member, MEK-2, resembles MEK-1 in its substrate specificity. MEK-3 (or MKK-3) functions to activate p38 MAP kinase, and MEK-4 (also called SEK1 or MKK-4) activates both p38 and JNK MAP kinases. MEK-5 appears to specifically phosphorylate ERK5, whereas MEK-6 phosphorylates p38 and p38b. MEK-7 (or MKK-7) phosphorylates and activates the JNK signal transduction pathway. |
| Immunogen | recombinant protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Recombinant rabbit |
| Tested Applications | WBICC/IF |
| WB:1:1,000-1:5,000 ICC:1:50-1:200 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Alternative Names | Dual specificity mitogen activated protein kinase kinase 5 antibody
Dual specificity mitogen-activated protein kinase kinase 5 antibody
EC 2.7.12.2 antibody
HsT17454 antibody
MAP kinase kinase 5 antibody
MAP kinase kinase MEK5b antibody
MAP2K5 antibody
MAPK/ERK kinase 5 antibody
MAPKK 5 antibody
MAPKK5 antibody
MEK 5 antibody
mitogen-activated protein kinase kinase 5 antibody
MKK5 antibody
MP2K5_HUMAN antibody
PRKMK5 antibody
Protein kinase mitogen-activated kinase 5 antibody SAPKK5 antibody SKK5 antibody |
|---|---|
| Molecular Weight(MW) | 49 kDa |
| Cellular Localization | Cytoplasm, Nucleus |
| SwissProt ID | Q13163 |
|---|
Application
Fig1: Western blot analysis of MEK5 on Hela cells lysates using anti-MEK5 antibody at 1/1,000 dilution.
Application
Fig2: ICC staining MEK5 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig3: ICC staining MEK5 in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig4: ICC staining MEK5 in SW480 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.| Positive Control | A431, SW480, Hela. |
|---|---|
| Application Notes | WB:1:1,000-1:5,000 ICC:1:50-1:200 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
