Application
Fig1: Western blot analysis of ATF5 on different lysates using anti-ATF5 antibody at 1/1,000 dilution. Positive control: Lane 1: Jurkat Lane 2: PC-12Application
Fig2: ICC staining ATF5 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig3: ICC staining ATF5 in PC-12 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig4: Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-ATF5 antibody. Counter stained with hematoxylin.Application
Fig5: Immunohistochemical analysis of paraffin-embedded human liver cancer tissue using anti-ATF5 antibody. Counter stained with hematoxylin.Application
Fig6: Immunohistochemical analysis of paraffin-embedded mouse stomach tissue using anti-ATF5 antibody. Counter stained with hematoxylin.Application
Fig7: Immunohistochemical analysis of paraffin-embedded mouse heart tissue using anti-ATF5 antibody. Counter stained with hematoxylin.| Product Name | ATF5 [SD2099] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Activating transcription factor 5 alpha/beta antibody Activating transcription factor 5 antibody Activating transcription factor X antibody AFTA antibody ATF 5 antibody ATF 7 antibody ATF7 antibody ATFX antibody BZIP protein ATF7 antibody cAMP dependent transcription factor ATF 5 antibody cAMP dependent transcription factor ATF5 antibody Cyclic AMP dependent transcription factor ATF 5 antibody Cyclic AMP dependent transcription factor ATF5 antibody FLJ34666 antibody HMFN0395 antibody MGC102397 antibody NAP1 antibody NRIF3 associated protein antibody ODA 10 antibody Transcription factor ATFx antibody Transcription factor like protein ODA 10 antibody Transcription factor like protein ODA10 antibody |
| Product description | Eukaryotic gene transcription is regulated by sequence-specific transcription factors that bind modular cis acting promoter and enhancer elements. The ATF/CREB transcription factor family binds the palindromic cAMP response element (CRE) octanucleotide TGACGTCA. The best characterized members of this gene family include CREB-1, CREB-2 (also designated ATF-4), CRE-BPa, LZIP (also designated CREB3 and Luman), CREM-1, CREM-2, ATF-1, ATF-2, ATF-3, ATF-5, ATF-6 and ATF-7. This family of proteins contain highly divergent N-terminal domains, but share a C-terminal leucine zipper for dimerization and DNA binding. ATF-5 (ATFx), which can localize to the cytoplasm or the nucleus, binds DNA as a dimer. It interacts with CCND3 and PTP4A1. |
| Immunogen | recombinant protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Recombinant rabbit |
| Tested Applications | WBICC/IFIHCIP |
| WB:1:1,000-1:2,000 ICC:1:50-1:200 IHC:1:50-1:200 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Alternative Names | Activating transcription factor 5 alpha/beta antibody Activating transcription factor 5 antibody Activating transcription factor X antibody AFTA antibody ATF 5 antibody ATF 7 antibody ATF7 antibody ATFX antibody BZIP protein ATF7 antibody cAMP dependent transcription factor ATF 5 antibody cAMP dependent transcription factor ATF5 antibody Cyclic AMP dependent transcription factor ATF 5 antibody Cyclic AMP dependent transcription factor ATF5 antibody FLJ34666 antibody HMFN0395 antibody MGC102397 antibody NAP1 antibody NRIF3 associated protein antibody ODA 10 antibody Transcription factor ATFx antibody Transcription factor like protein ODA 10 antibody Transcription factor like protein ODA10 antibody |
|---|---|
| Molecular Weight(MW) | 31 kDa |
| Cellular Localization | Cytoplasm, Nucleus. |
Application
Fig1: Western blot analysis of ATF5 on different lysates using anti-ATF5 antibody at 1/1,000 dilution. Positive control: Lane 1: Jurkat Lane 2: PC-12
Application
Fig2: ICC staining ATF5 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig3: ICC staining ATF5 in PC-12 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig4: Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-ATF5 antibody. Counter stained with hematoxylin.
Application
Fig5: Immunohistochemical analysis of paraffin-embedded human liver cancer tissue using anti-ATF5 antibody. Counter stained with hematoxylin.
Application
Fig6: Immunohistochemical analysis of paraffin-embedded mouse stomach tissue using anti-ATF5 antibody. Counter stained with hematoxylin.
Application
Fig7: Immunohistochemical analysis of paraffin-embedded mouse heart tissue using anti-ATF5 antibody. Counter stained with hematoxylin.| Positive Control | PC-12, Jurkat, Hela, human tonsil tissue, mouse stomach tissue, human liver cancer tissue, mouse heart tissue. |
|---|---|
| Application Notes | WB:1:1,000-1:2,000 ICC:1:50-1:200 IHC:1:50-1:200 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
