Application
Fig1: Western blot analysis of HDAC4 on different lysates using anti-HDAC4 antibody at 1/1,000 dilution. Positive control: Lane 1: Hela Lane 2: HepG2Application
Fig2: ICC staining HDAC4 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig3: ICC staining HDAC4 in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig4: ICC staining HDAC4 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.| Product Name | HDAC4 [SD08-35] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | AHO3 antibody BDMR antibody EC 3.5.1.98 antibody HA6116 antibody HD 4 antibody HD4 antibody HDAC 4 antibody HDAC A antibody HDAC4 antibody HDAC4_HUMAN antibody HDACA antibody Histone deacetylase 4 antibody Histone Deacetylase A antibody KIAA0288 antibody |
| Product description | In the intact cell, DNA closely associates with histones and other nuclear proteins to form chromatin. The remodeling of chromatin is believed to be a critical component of transcriptional regulation and a major source of this remodeling is brought about by the acetylation of nucleosomal histones. Acetylation of lysine residues in the amino terminal tail domain of histone results in an allosteric change in the nucleosomal conformation and an increased accessibility to transcription factors by DNA. Conversely, the deacetylation of histones is associated with transcriptional silencing. Several mammalian proteins have been identified as nuclear histone acetylases, including GCN5, p300/CBP, PCAF (p300/CBPassociated factor), HAT1, and the TFIID subunit TAF II p250. Mammalian HDAC1 (also designated HD1), HDAC2 (also designated RPD3) and HDAC3-6, have been identified as histone deacetylases. |
| Immunogen | recombinant protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Recombinant rabbit |
| Tested Applications | WBICC/IF |
| WB:1:1,000-1:2,000 ICC:1:100-1:500 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Alternative Names | AHO3 antibody BDMR antibody EC 3.5.1.98 antibody HA6116 antibody HD 4 antibody HD4 antibody HDAC 4 antibody HDAC A antibody HDAC4 antibody HDAC4_HUMAN antibody HDACA antibody Histone deacetylase 4 antibody Histone Deacetylase A antibody KIAA0288 antibody |
|---|---|
| Molecular Weight(MW) | 120 kDa |
| Cellular Localization | Nucleus, Cytoplasm. |
| SwissProt ID | P56524 |
|---|
Application
Fig1: Western blot analysis of HDAC4 on different lysates using anti-HDAC4 antibody at 1/1,000 dilution. Positive control: Lane 1: Hela Lane 2: HepG2
Application
Fig2: ICC staining HDAC4 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig3: ICC staining HDAC4 in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig4: ICC staining HDAC4 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.| Positive Control | A549, Hela, HepG2. |
|---|---|
| Application Notes | WB:1:1,000-1:2,000 ICC:1:100-1:500 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
