Application
Fig1: Western blot analysis of AMPK beta 1 on A431 cells lysates using anti-AMPK beta 1 antibody at 1/1,000 dilution.Application
Fig2: Flow cytometric analysis of A431 cells with AMPK beta 1 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody| Product Name | AMPK beta 1 [SD082-06] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | 1300015D22Rik antibody 5''-AMP-activated protein kinase subunit beta-1 antibody 5'-AMP-activated protein kinase beta-1 subunit antibody AAKB1_HUMAN antibody AMP-activated protein kinase beta subunit antibody AMP-ACTIVATED PROTEIN KINASE, NONCATALYTIC, BETA-1 antibody AMP-activated, noncatalytic, beta-1 antibody AMPK antibody AMPK beta 1 chain antibody AMPK subunit beta-1 antibody AMPK-BETA-1 antibody AMPKb antibody AU021155 antibody E430008F22 antibody HAMPKb antibody MGC17785 antibody PRKAB1 antibody Protein kinase AMP activated non catalytic subunit beta 1 antibody protein kinase, AMP-activated, beta 1 non-catalytic subunit antibody protein kinase, AMP-activated, noncatalytic, beta-1 antibody |
| Product description | AMPK (for 5'-AMP-activated protein kinase) is a heterotrimeric complex com-prising a catalytic α subunit and regulatory β and γ subunits. It protects cells from stresses that cause ATP depletion by switching off ATP-consuming bio-synthetic pathways. AMPK is activated by high AMP and low ATP through a mechanism involving allosteric regulation, promotion of phosphorylation by an upstream protein kinase known as AMPK kinase, and inhibition of dephosphorylation. Activated AMPK can phosphorylate and regulate in vivo hydroxy-methylglutaryl-CoA reductase and acetyl-CoA carboxylase, which are key regulatory enzymes of sterol synthesis and fatty acid synthesis, respectively. The human AMPKα1 and AMPKα2 genes encode 548 amino acid and 552 amino acid proteins, respectively. Human AMPKβ1 encodes a 271 amino acid protein and human AMPKβ2 encodes a 272 amino acid protein. The human AMPKγ1 gene encodes a 331 amino acid protein. Human AMPKγ2 and AMPKγ3, which are 569 and 492 amino acid proteins, respectively, contain unique N-terminal domains and may participate directly in the binding of AMP within the AMPK complex. |
| Immunogen | recombinant protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Recombinant rabbit |
| Tested Applications | WBFC |
| WB:1:1,000-1:2,000 FC:1:50-1:100 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Alternative Names | 1300015D22Rik antibody
5''-AMP-activated protein kinase subunit beta-1 antibody
5'-AMP-activated protein kinase beta-1 subunit antibody
AAKB1_HUMAN antibody
AMP-activated protein kinase beta subunit antibody
AMP-ACTIVATED PROTEIN KINASE NONCATALYTIC BETA-1 antibody AMP-activated noncatalytic beta-1 antibody AMPK antibody AMPK beta 1 chain antibody AMPK subunit beta-1 antibody AMPK-BETA-1 antibody AMPKb antibody AU021155 antibody E430008F22 antibody HAMPKb antibody MGC17785 antibody PRKAB1 antibody Protein kinase AMP activated non catalytic subunit beta 1 antibody protein kinase AMP-activated beta 1 non-catalytic subunit antibody protein kinase AMP-activated noncatalytic beta-1 antibody |
|---|---|
| Molecular Weight(MW) | 38 kDa |
| Cellular Localization | Cytosol, Nucleoplasm. |
Application
Fig1: Western blot analysis of AMPK beta 1 on A431 cells lysates using anti-AMPK beta 1 antibody at 1/1,000 dilution.
Application
Fig2: Flow cytometric analysis of A431 cells with AMPK beta 1 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody| Positive Control | A431. |
|---|---|
| Application Notes | WB:1:1,000-1:2,000 FC:1:50-1:100 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
