Application
Fig1: Western blot analysis of JunB on different lysates using anti-JunB antibody at 1/1,000 dilution. Positive control: Lane 1: U937 Lane 2: THP-1Application
Fig2: ICC staining JunB in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig3: ICC staining JunB in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.| Product Name | JunB [SD081-08] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Activator protein 1 antibody AP 1 antibody AP1 antibody Jun B antibody Jun B proto oncogene antibody Jun B protooncogene antibody Junb antibody JunB proto oncogene antibody JunB protoncogene 9 antibody JunB protooncogene antibody JUNB_HUMAN antibody Transcription factor jun B antibody Transcription factor jun-B antibody Transcription factor junB antibody |
| Product description | The c-Jun proto-oncogene was first identified as the cellular homolog of the avian sarcoma virus v-Jun oncogene. The c-Jun protein along with c-Fos is a component of the AP-1 transcriptional complex. c-Jun can form either Jun/Jun homodimers or Jun/Fos heterodimers via the leucine repeats in both proteins. Homo- and heterodimers bind to the TGACTCA consensus sequence present in numerous promoters and initially identified as the phorbol ester tumor promoter response element (TRE). Two additional genes, Jun B and Jun D have been shown to be almost identical to c-Jun in their C-terminal regions, which are involved in dimerization and DNA binding, whereas their N-terminal domains, which are involved in transcriptional activation, diverge. All three form heterodimers among themselves and with c-Fos and other members of the Fos gene family. |
| Immunogen | recombinant protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Recombinant rabbit |
| Tested Applications | WBICC/IFIP |
| WB:1:1,000-1:2,000 ICC:1:50-1:200 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Alternative Names | Activator protein 1 antibody AP 1 antibody AP1 antibody Jun B antibody Jun B proto oncogene antibody Jun B protooncogene antibody Junb antibody JunB proto oncogene antibody JunB protoncogene 9 antibody JunB protooncogene antibody JUNB_HUMAN antibody Transcription factor jun B antibody Transcription factor jun-B antibody Transcription factor junB antibody |
|---|---|
| Molecular Weight(MW) | 43 kDa |
| Cellular Localization | Nucleus. |
Application
Fig1: Western blot analysis of JunB on different lysates using anti-JunB antibody at 1/1,000 dilution. Positive control: Lane 1: U937 Lane 2: THP-1
Application
Fig2: ICC staining JunB in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig3: ICC staining JunB in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.| Positive Control | U937, THP-1, Hela, HepG2. |
|---|---|
| Application Notes | WB:1:1,000-1:2,000 ICC:1:50-1:200 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
