Application
Fig1: Western blot analysis of ERK5 on Hela cells lysates using anti-ERK5 antibody at 1/1,000 dilution.Application
Fig2: ICC staining ERK5 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig3: ICC staining ERK5 in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig4: ICC staining ERK5 in 293 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig5: Flow cytometric analysis of A549 cells with ERK5 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.| Product Name | ERK5 [SD2084] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Big MAP kinase 1 antibody BMK 1 antibody BMK 1 kinase antibody BMK-1 antibody BMK1 antibody BMK1 Kinase antibody EC 2.7.11.24 antibody ERK 4 antibody ERK 5 antibody ERK-5 antibody ERK4 antibody ERK5 antibody Extracellular signal regulated kinase 5 antibody Extracellular signal-regulated kinase 5 antibody MAP kinase 7 antibody MAPK 7 antibody MAPK7 antibody Mitogen activated protein kinase 7 antibody Mitogen-activated protein kinase 7 antibody MK07_HUMAN antibody OTTHUMP00000065906 antibody OTTHUMP00000065907 antibody PRKM 7 antibody PRKM7 antibody PROTEIN KINASE, MITOGEN-ACTIVATED, 7 antibody |
| Product description | The activation of signal transduction pathways by growth factors, hormones and neurotransmitters is mediated through two closely related MAP kinases, p44 and p42, designated extracellular-signal related kinase 1 (ERK 1) and ERK 2, respectively. ERK proteins are regulated by dual phosphorylation at specific tyrosine and threonine sites mapping within a characteristic Thr-Glu-Tyr motif. Phosphorylation at both the Thr and Tyr residues is required for full enzymatic activation. In response to activation, MAP kinases phosphorylate downstream components on serine and threonine. Upstream MAP kinase regulators include MAP kinase kinase (MEK), MEK kinase and Raf-1. The ERK family has three additional members: ERK 3, ERK 5 and ERK 6. |
| Immunogen | recombinant protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Recombinant rabbit |
| Tested Applications | WBICC/IFIPFC |
| WB:1:1,000-1:2,000 ICC:1:100-1:500 FC:1:50-1:100 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Alternative Names | Big MAP kinase 1 antibody
BMK 1 antibody
BMK 1 kinase antibody
BMK-1 antibody
BMK1 antibody
BMK1 Kinase antibody
EC 2.7.11.24 antibody
ERK 4 antibody
ERK 5 antibody
ERK-5 antibody
ERK4 antibody
ERK5 antibody
Extracellular signal regulated kinase 5 antibody
Extracellular signal-regulated kinase 5 antibody
MAP kinase 7 antibody
MAPK 7 antibody
MAPK7 antibody
Mitogen activated protein kinase 7 antibody
Mitogen-activated protein kinase 7 antibody
MK07_HUMAN antibody
OTTHUMP00000065906 antibody
OTTHUMP00000065907 antibody
PRKM 7 antibody
PRKM7 antibody
PROTEIN KINASE MITOGEN-ACTIVATED 7 antibody |
|---|---|
| Molecular Weight(MW) | 115 kDa |
| Cellular Localization | Cytoplasm, Nucleus. |
Application
Fig1: Western blot analysis of ERK5 on Hela cells lysates using anti-ERK5 antibody at 1/1,000 dilution.
Application
Fig2: ICC staining ERK5 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig3: ICC staining ERK5 in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig4: ICC staining ERK5 in 293 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig5: Flow cytometric analysis of A549 cells with ERK5 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.| Positive Control | MCF-7, Hela, 293. |
|---|---|
| Application Notes | WB:1:1,000-1:2,000 ICC:1:100-1:500 FC:1:50-1:100 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
