Application
Fig1: Western blot analysis of HSPA4 on Hela cells lysates using anti-HSPA4 antibody at 1/1,000 dilution.Application
Fig2: ICC staining HSPA4 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig3: ICC staining HSPA4 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig4: Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-HSPA4 antibody. Counter stained with hematoxylin.Application
Fig5: Immunohistochemical analysis of paraffin-embedded human liver cancer tissue using anti-HSPA4 antibody. Counter stained with hematoxylin.Application
Fig6: Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-HSPA4 antibody. Counter stained with hematoxylin.Application
Fig7: Flow cytometric analysis of Hela cells with HSPA4 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.| Product Name | HSPA4 [SD0861] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | APG 2 antibody Epididymis secretory sperm binding protein Li 5a antibody Heat shock 70 kDa protein 4 antibody Heat shock 70 related protein APG 2 antibody Heat shock 70-related protein APG-2 antibody Heat shock protein, 110 kDa antibody HEL S 5a antibody HS24/P52 antibody hsp70 antibody Hsp70 RY antibody hsp70RY antibody HSP74_HUMAN antibody HSPA4 antibody HSPH2 antibody MGC131852 antibody RY antibody |
| Product description | The heat shock proteins (HSPs) comprise a group of highly conserved, abundantly expressed proteins with diverse functions, which include the assembly and sequestering of multiprotein complexes, transportation of nascent polypeptide chains across cellular membranes and regulation of protein folding. Heat shock proteins (also known as molecular chaperones) fall into six general families: HSP 90, HSP 70, HSP 60, the low molecular weight HSPs, the immunophilins and the HSP 110 family. The HSP 110 family (also known as the HSP 105 family) is composed of HSP 105, Apg-1 and Apg-2. |
| Immunogen | recombinant protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Recombinant rabbit |
| Tested Applications | WBICC/IFIHCFC |
| WB:1:1,000-1:5,000 ICC:1:100-1:500 IHC:1:50-1:200 FC:1:50-1:100 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Alternative Names | APG 2 antibody
Epididymis secretory sperm binding protein Li 5a antibody
Heat shock 70 kDa protein 4 antibody
Heat shock 70 related protein APG 2 antibody
Heat shock 70-related protein APG-2 antibody
Heat shock protein 110 kDa antibody HEL S 5a antibody HS24/P52 antibody hsp70 antibody Hsp70 RY antibody hsp70RY antibody HSP74_HUMAN antibody HSPA4 antibody HSPH2 antibody MGC131852 antibody RY antibody |
|---|---|
| Molecular Weight(MW) | 94 kDa |
| Cellular Localization | Cytoplasm, Nucleus. |
Application
Fig1: Western blot analysis of HSPA4 on Hela cells lysates using anti-HSPA4 antibody at 1/1,000 dilution.
Application
Fig2: ICC staining HSPA4 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig3: ICC staining HSPA4 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig4: Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-HSPA4 antibody. Counter stained with hematoxylin.
Application
Fig5: Immunohistochemical analysis of paraffin-embedded human liver cancer tissue using anti-HSPA4 antibody. Counter stained with hematoxylin.
Application
Fig6: Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-HSPA4 antibody. Counter stained with hematoxylin.
Application
Fig7: Flow cytometric analysis of Hela cells with HSPA4 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.| Positive Control | Hela, HepG2, rat brain tissue, mouse brain tissue, human liver cancer tissue. |
|---|---|
| Application Notes | WB:1:1,000-1:5,000 ICC:1:100-1:500 IHC:1:50-1:200 FC:1:50-1:100 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
