Application
Fig1: Western blot analysis of CDC40 on Hela cells lysates using anti-CDC40 antibody at 1/2,000 dilution.Application
Fig2: ICC staining CDC40 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig3: ICC staining CDC40 in HUVEC cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig4: ICC staining CDC40 in SHG-44 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig5: Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-CDC40 antibody. Counter stained with hematoxylin.Application
Fig6: Immunohistochemical analysis of paraffin-embedded mouse colon tissue using anti-CDC40 antibody. Counter stained with hematoxylin.Application
Fig7: Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-CDC40 antibody. Counter stained with hematoxylin.Application
Fig8: Flow cytometric analysis of K562 cells with CDC40 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.| Product Name | CDC40 [SD085-9] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Cdc40 antibody Cell division cycle 40 antibody Cell division cycle 40 homolog antibody EH binding protein 3 antibody EH-binding protein 3 antibody Ehb3 antibody hPRP17 antibody Pre mRNA processing factor 17 antibody Pre mRNA splicing factor 17 antibody Pre-mRNA-processing factor 17 antibody PRP17 antibody PRP17 homolog antibody PRP17_HUMAN antibody PRPF17 antibody |
| Product description | Cell cycle events are regulated by the sequential activation and deactivation of cyclin dependent kinases (Cdks) and by the proteolysis of cyclins. The cell division control (Cdc) genes are required at various points in the cell cycle. Cdc40, also known as pre-mRNA-processing factor 17 (PRPF17) or EH-binding protein 3 (EHB3), is a 579 amino acid nuclear protein. Cdc40 is essential for the catalytic step II of the pre-mRNA splicing process, in which Cdc40 associates with the spliceosome C complex. Cdc40 contains seven WD repeats, which are important in protein-protein interactions. Cdc40 has sequence similarity to the yeast protein Prp17, which is involved in pre-mRNA splicing and cell cycle progression. The sequence similarity between the mammalian Cdc40 and the yeast Prp17 may indicate an additional role in cell cycle progression for mammalian Cdc40. |
| Immunogen | recombinant protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Recombinant rabbit |
| Tested Applications | WBICC/IFIHCFC |
| WB:1:1,000-1:5,000 ICC:1:50-1:200 IHC:1:50-1:200 FC:1:50-1:100 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Alternative Names | Cdc40 antibody Cell division cycle 40 antibody Cell division cycle 40 homolog antibody EH binding protein 3 antibody EH-binding protein 3 antibody Ehb3 antibody hPRP17 antibody Pre mRNA processing factor 17 antibody Pre mRNA splicing factor 17 antibody Pre-mRNA-processing factor 17 antibody PRP17 antibody PRP17 homolog antibody PRP17_HUMAN antibody PRPF17 antibody |
|---|---|
| Molecular Weight(MW) | 66 kDa |
| Cellular Localization | Nucleus. |
Application
Fig1: Western blot analysis of CDC40 on Hela cells lysates using anti-CDC40 antibody at 1/2,000 dilution.
Application
Fig2: ICC staining CDC40 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig3: ICC staining CDC40 in HUVEC cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig4: ICC staining CDC40 in SHG-44 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig5: Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-CDC40 antibody. Counter stained with hematoxylin.
Application
Fig6: Immunohistochemical analysis of paraffin-embedded mouse colon tissue using anti-CDC40 antibody. Counter stained with hematoxylin.
Application
Fig7: Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-CDC40 antibody. Counter stained with hematoxylin.
Application
Fig8: Flow cytometric analysis of K562 cells with CDC40 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.| Positive Control | HUVEC, SHG-44, Hela, mouse brain tissue, rat brain tissue, mouse colon tissue. |
|---|---|
| Application Notes | WB:1:1,000-1:5,000 ICC:1:50-1:200 IHC:1:50-1:200 FC:1:50-1:100 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
