Application
Fig1: Western blot analysis of Phospho-Raf1(S259) on different lysates using anti-Phospho-Raf1(S259) antibody at 1/1,000 dilution. Positive control: Lane 1: Human skeletal muscle Lane 2: HelaApplication
Fig2: Immunohistochemical analysis of paraffin-embedded rat skeletal muscle tissue using anti-Phospho-Raf1(S259) antibody. Counter stained with hematoxylin.| Product Name | Phospho-Raf1 (S259) [SD85-07] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | c Raf antibody C-raf antibody C-Raf proto-oncogene, serine/threonine kinase antibody CMD1NN antibody Craf 1 transforming gene antibody cRaf antibody Craf1 transforming gene antibody EC 2.7.11.1 antibody kinase Raf1 antibody Murine sarcoma 3611 oncogene 1 antibody NS5 antibody Oncogene MIL antibody Oncogene RAF1 antibody OTTHUMP00000160218 antibody OTTHUMP00000207813 antibody OTTHUMP00000209389 antibody Protein kinase raf 1 antibody Proto-oncogene c-RAF antibody Raf 1 antibody Raf 1 proto oncogene serine/threonine kinase antibody RAF antibody Raf proto oncogene serine/threonine protein kinase antibody RAF proto-oncogene serine/threonine-protein kinase antibody RAF-1 antibody RAF1 antibody RAF1_HUMAN antibody Similar to murine leukemia viral (V-raf-1) oncogene homolog 1 antibody TRANSFORMING REPLICATION-DEFECTIVE MURINE RETROVIRUS 3611-MSV antibody v raf 1 murine leukemia viral oncogene homolog 1 antibody v-raf murine sarcoma viral oncogene homolog 1 antibody v-raf-1 murine leukemia viral oncogene-like protein 1 antibody vraf1 murine leukemia viral oncogene homolog 1 antibody |
| Product description | Raf-1 is a ubiquitously expressed cytoplasmic protein with intrinsic serine/threonine kinase activity. Raf-1, or c-Raf, is the cellular homolog of v-Raf, the product of the transforming gene of the 3611 strain of murine sarcoma virus. The unregulated kinase activity of the v-Raf protein is associated with cellular transformation and mitogenesis. Raf-1 is normally suppressed by its regulatory N-terminal domain. Raf-1 is activated in response to a variety of tyrosine kinase receptors as well as in response to pp60v-Src expression. Specifically, Raf-1 is phosphorylated in the catalytic domain at Ser 338 and, to a lesser extent, Ser 339. This phosphorylation requires the co-activation of PI 3-kinase and the Ras signaling pathway. Raf-1 is also phosphorylated on Tyr 340 and 341, which induces the phosphorylation of MEK. Phosphorylation of Ser 621 is essential for the catalytic activity of Raf-1 and downregulation by c-AMP-dependent protein kinase A (PKA). PKA also phosphorylates Raf-1 on Ser 43 and Ser 259. PKA phosphorylation of Ser 259 inhibits Raf-1 and decreases the phosphorylation necessary for Raf-1 activation at Ser 338. |
| Immunogen | Synthetic phospho-peptide corresponding to residues surrounding Ser259 of human Raf1 |
| Modification | p-S259 |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Recombinant rabbit |
| Tested Applications | WBIHC |
| WB:1:1,000-1:2,000 IHC:1:50-1:200 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Alternative Names | c Raf antibody
C-raf antibody
C-Raf proto-oncogene serine/threonine kinase antibody CMD1NN antibody Craf 1 transforming gene antibody cRaf antibody Craf1 transforming gene antibody EC 2.7.11.1 antibody kinase Raf1 antibody Murine sarcoma 3611 oncogene 1 antibody NS5 antibody Oncogene MIL antibody Oncogene RAF1 antibody OTTHUMP00000160218 antibody OTTHUMP00000207813 antibody OTTHUMP00000209389 antibody Protein kinase raf 1 antibody Proto-oncogene c-RAF antibody Raf 1 antibody Raf 1 proto oncogene serine/threonine kinase antibody RAF antibody Raf proto oncogene serine/threonine protein kinase antibody RAF proto-oncogene serine/threonine-protein kinase antibody RAF-1 antibody RAF1 antibody RAF1_HUMAN antibody Similar to murine leukemia viral (V-raf-1) oncogene homolog 1 antibody TRANSFORMING REPLICATION-DEFECTIVE MURINE RETROVIRUS 3611-MSV antibody v raf 1 murine leukemia viral oncogene homolog 1 antibody v-raf murine sarcoma viral oncogene homolog 1 antibody v-raf-1 murine leukemia viral oncogene-like protein 1 antibody vraf1 murine leukemia viral oncogene homolog 1 antibody |
|---|---|
| Molecular Weight(MW) | 73 kDa |
| Cellular Localization | Cytoplasm, Cell membrane, Mitochondrion, Nucleus. |
Application
Fig1: Western blot analysis of Phospho-Raf1(S259) on different lysates using anti-Phospho-Raf1(S259) antibody at 1/1,000 dilution. Positive control: Lane 1: Human skeletal muscle Lane 2: Hela
Application
Fig2: Immunohistochemical analysis of paraffin-embedded rat skeletal muscle tissue using anti-Phospho-Raf1(S259) antibody. Counter stained with hematoxylin.| Positive Control | Hela, human skeletal muscle tissue. |
|---|---|
| Application Notes | WB:1:1,000-1:2,000 IHC:1:50-1:200 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
