Application
Fig1: Western blot analysis of 14-3-3 gamma on different lysates using anti-14-3-3 gamma antibody at 1/1,000 dilution. Positive control: Lane 1: 293T Lane 2: A431 Lane 3: HelaApplication
Fig2: Flow cytometric analysis of K562 cells with 14-3-3 gamma antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the sec?皪?皫??皩?羉瘍| Product Name | 14-3-3 gamma [SD20-65] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | 14 3 3 gamma antibody 14 3 3 protein gamma antibody 14 3 3 protein gamma subtype antibody 14 3 3gamma antibody 14-3-3 protein gamma antibody 1433G_HUMAN antibody 3 monooxygenase/tryptophan 5 monooxgenase activation protein gamma polypeptide antibody KCIP 1 antibody KCIP-1 antibody KCIP1 antibody N-terminally processed antibody Protein kinase C inhibitor protein 1 antibody Tyrosine 3 monooxygenase/tryptophan 5 monooxygenase activation protein gamma polypeptide antibody Ywhag antibody |
| Product description | 14-3-3 proteins regulate many cellular processes relevant to cancer biology, notably apoptosis, mitogenic signaling and cell-cycle checkpoints. Seven isoforms comprise this family of signaling intermediates, denoted 14-3-3 b, g, e, z, h, q and s. 14-3-3 proteins form dimers that present two binding sites for ligand proteins, thereby bringing together two proteins that may not otherwise associate. These ligands largely share a 14-3-3 consensus binding motif and exhibit serine/threonine phosphorylation. 14-3-3 proteins function in broad regulation of these ligand proteins; by cytoplasmic sequestration, occupation of interaction domains and import/export sequences, prevention of degradation, activation/repression of enzymatic activity, and facilitation of protein modification. Loss of expression contributes to a vast array of pathogenic cellular activities. |
| Immunogen | recombinant protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Recombinant rabbit |
| Tested Applications | WBFC |
| WB:1:1,000-1:2,000 FC:1:10-1:50 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Alternative Names | 14 3 3 gamma antibody 14 3 3 protein gamma antibody 14 3 3 protein gamma subtype antibody 14 3 3gamma antibody 14-3-3 protein gamma antibody 1433G_HUMAN antibody 3 monooxygenase/tryptophan 5 monooxgenase activation protein gamma polypeptide antibody KCIP 1 antibody KCIP-1 antibody KCIP1 antibody N-terminally processed antibody Protein kinase C inhibitor protein 1 antibody Tyrosine 3 monooxygenase/tryptophan 5 monooxygenase activation protein gamma polypeptide antibody Ywhag antibody |
|---|---|
| Molecular Weight(MW) | 28 kDa |
| Cellular Localization | Cytoplasm. |
Application
Fig1: Western blot analysis of 14-3-3 gamma on different lysates using anti-14-3-3 gamma antibody at 1/1,000 dilution. Positive control: Lane 1: 293T Lane 2: A431 Lane 3: Hela
Application
Fig2: Flow cytometric analysis of K562 cells with 14-3-3 gamma antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the sec?皪?皫??皩?羉瘍| Positive Control | A431, Hela, 293T. |
|---|---|
| Application Notes | WB:1:1,000-1:2,000 FC:1:10-1:50 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
