Application
Fig1: Western blot analysis of Collagen VI on different lysates using anti-Collagen VI antibody at 1/1,000 dilution. Positive control: Lane 1: Mouse heart Lane 2: Human heart Lane 3: Human kidneyApplication
Fig2: ICC staining Collagen VI in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig3: ICC staining Collagen VI in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig4: ICC staining Collagen VI in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig5: ICC staining Collagen VI in RH-35 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig6: Immunohistochemical analysis of paraffin-embedded human lung tissue using anti-Collagen VI antibody. Counter stained with hematoxylin.Application
Fig7: Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-Collagen VI antibody. Counter stained with hematoxylin.Application
Fig8: Immunohistochemical analysis of paraffin-embedded mouse colon tissue using anti-Collagen VI antibody. Counter stained with hematoxylin.| Product Name | Collagen VI [SD83-03] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Alpha 1 (VI) chain (61 AA) antibody CO6A1_HUMAN antibody COL6A1 antibody COL6A2 antibody COL6A3 antibody Collagen alpha 2(VI) chain antibody Collagen alpha 3(VI) chain antibody Collagen alpha-1(VI) chain antibody Collagen type VI alpha 1 antibody Collagen type VI alpha 2 antibody Collagen type VI alpha 3 antibody Collagen VI alpha 1 polypeptide antibody Collagen VI alpha 2 polypeptide antibody Collagen VI alpha 3 polypeptide antibody CollagenVI antibody Human mRNA for collagen VI alpha 2 C terminal globular domain antibody OPLL antibody PP3610 antibody |
| Product description | This antibody is well suited to detect extracellular matrix proteins in normal as well as disease state tissues. Disruption of tissue organization is the hallmark of neoplasia. Malignant lesions can be distinguished from benign by examining the breakdown of basement membranes and loss of 3-dimensional architecture. Malignant cells are presumed to use matrix metalloproteases to degrade barriers created by the extracellular matrix which then allows metastasis to occur. Collagenases, stomelysins and gelatinases can collectively degrade all of the various components of the extracellular matrix, including fibrillar and non-fibrillar collagens and basement membrane glycoproteins. |
| Immunogen | recombinant protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Recombinant rabbit |
| Tested Applications | WBICC/IFIHC |
| WB:1:1,000-1:2,000 ICC:1:100-1:500 IHC:1:50-1:200 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Alternative Names | Alpha 1 (VI) chain (61 AA) antibody CO6A1_HUMAN antibody COL6A1 antibody COL6A2 antibody COL6A3 antibody Collagen alpha 2(VI) chain antibody Collagen alpha 3(VI) chain antibody Collagen alpha-1(VI) chain antibody Collagen type VI alpha 1 antibody Collagen type VI alpha 2 antibody Collagen type VI alpha 3 antibody Collagen VI alpha 1 polypeptide antibody Collagen VI alpha 2 polypeptide antibody Collagen VI alpha 3 polypeptide antibody CollagenVI antibody Human mRNA for collagen VI alpha 2 C terminal globular domain antibody OPLL antibody PP3610 antibody |
|---|---|
| Molecular Weight(MW) | 134/109 kDa |
| Cellular Localization | Secreted, Membrane. |
Application
Fig1: Western blot analysis of Collagen VI on different lysates using anti-Collagen VI antibody at 1/1,000 dilution. Positive control: Lane 1: Mouse heart Lane 2: Human heart Lane 3: Human kidney
Application
Fig2: ICC staining Collagen VI in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig3: ICC staining Collagen VI in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig4: ICC staining Collagen VI in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig5: ICC staining Collagen VI in RH-35 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig6: Immunohistochemical analysis of paraffin-embedded human lung tissue using anti-Collagen VI antibody. Counter stained with hematoxylin.
Application
Fig7: Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-Collagen VI antibody. Counter stained with hematoxylin.
Application
Fig8: Immunohistochemical analysis of paraffin-embedded mouse colon tissue using anti-Collagen VI antibody. Counter stained with hematoxylin.| Positive Control | A549, RH-35, Hela, HepG2, human liver tissue, mouse colon tissue, human lung tissue, human heart tissue, human kidney tissue, mouse heart tissue. |
|---|---|
| Application Notes | WB:1:1,000-1:2,000 ICC:1:100-1:500 IHC:1:50-1:200 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
