Application
Fig1: Western blot analysis of MEKK3 on different lysates using anti-MEKK3 antibody at 1/1,000 dilution. Positive control: Lane 1: Hela Lane 2: 293T Lane 3: A431Application
Fig2: ICC staining MEKK3 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig3: ICC staining MEKK3 in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig4: ICC staining MEKK3 in SKOV-3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig5: Immunohistochemical analysis of paraffin-embedded mouse uterus tissue using anti-MEKK3 antibody. Counter stained with hematoxylin.Application
Fig6: Immunohistochemical analysis of paraffin-embedded rat uterus tissue using anti-MEKK3 antibody. Counter stained with hematoxylin.Application
Fig7: Flow cytometric analysis of Hela cells with MEKK3 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.| Product Name | MEKK3 [SD0839] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | M3K3_HUMAN antibody MAP/ERK kinase kinase 3 antibody MAP3K3 antibody MAPK/ERK kinase kinase 3 antibody MAPKKK3 antibody MEK kinase 3 antibody MEKK 3 antibody MEKK3 antibody Mitogen activated protein kinase kinase kinase 3 antibody Mitogen-activated protein kinase kinase kinase 3 antibody |
| Product description | MAP kinase kinase kinase (MEKK3 or MAP3K3) is a serine/threonine protein kinase that activates SAPK and ERK via phosphorylation and activation of their respective MAP kinase kinases, SEK and MEK1/2. MEKK3 also stimulates MEK5 via activation of ERK5/BMK1, which is at least partly regulated by a direct interaction between MEK5 and MEKK3 via p67phox-Bem1p (PB1) protein-protein interaction domains found in both proteins. MEKK3 modulates NF-κB activation in response to a variety of agonists including TNFα, LPS, IL-1 and LPA. Despite reports showing that phosphorylation of MEKK3 at Ser526 within the activation loop is necessary for kinase activation, at least one study suggests that dual phosphorylation at Thr516 and Ser520 is required for LPA-stimulated IKKβ/NF-κB activation. Phosphorylation at Thr294 appears to negatively regulate MEKK3 by promoting 14-3-3β binding and inhibition of the kinase activity. |
| Immunogen | recombinant protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Recombinant rabbit |
| Tested Applications | WBICC/IFIHCIPFC |
| WB:1:1,000-1:2,000 ICC:1:100-1:500 IHC:1:50-1:200 FC:1:50-1:100 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Alternative Names | M3K3_HUMAN antibody MAP/ERK kinase kinase 3 antibody MAP3K3 antibody MAPK/ERK kinase kinase 3 antibody MAPKKK3 antibody MEK kinase 3 antibody MEKK 3 antibody MEKK3 antibody Mitogen activated protein kinase kinase kinase 3 antibody Mitogen-activated protein kinase kinase kinase 3 antibody |
|---|---|
| Molecular Weight(MW) | 71 kDa |
| Cellular Localization | Cytoplasm. |
Application
Fig1: Western blot analysis of MEKK3 on different lysates using anti-MEKK3 antibody at 1/1,000 dilution. Positive control: Lane 1: Hela Lane 2: 293T Lane 3: A431
Application
Fig2: ICC staining MEKK3 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig3: ICC staining MEKK3 in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig4: ICC staining MEKK3 in SKOV-3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig5: Immunohistochemical analysis of paraffin-embedded mouse uterus tissue using anti-MEKK3 antibody. Counter stained with hematoxylin.
Application
Fig6: Immunohistochemical analysis of paraffin-embedded rat uterus tissue using anti-MEKK3 antibody. Counter stained with hematoxylin.
Application
Fig7: Flow cytometric analysis of Hela cells with MEKK3 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.| Positive Control | A431, 293T, MCF-7, Hela, SKOV-3, rat uterus tissue, mouse uterus tissue. |
|---|---|
| Application Notes | WB:1:1,000-1:2,000 ICC:1:100-1:500 IHC:1:50-1:200 FC:1:50-1:100 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
