Application
Fig1: Western blot analysis of Sumo 2+3 on SW480 cells lysates using anti- Sumo 2+3 antibody at 1/1,000 dilution.Application
Fig2: ICC staining Sumo 2+3 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig3: ICC staining Sumo 2+3 in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig4: ICC staining Sumo 2+3 in RH-35 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig5: Immunohistochemical analysis of paraffin-embedded mouse lung tissue using anti- Sumo 2+3 antibody. Counter stained with hematoxylin.Application
Fig6: Immunohistochemical analysis of paraffin-embedded mouse skin tissue using anti- Sumo 2+3 antibody. Counter stained with hematoxylin.Application
Fig7: Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti- Sumo 2+3 antibody. Counter stained with hematoxylin.Application
Fig8: Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using anti- Sumo 2+3 antibody. Counter stained with hematoxylin.| Product Name | Sumo 2+3 [JJ087-04] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | HSMT3 antibody MGC117191 antibody OTTHUMP00000115275 antibody OTTHUMP00000115276 antibody OTTHUMP00000115277 antibody Sentrin 2 antibody Small ubiquitin like modifier 2 antibody Small ubiquitin related modifier 2 antibody small ubiquitin-like modifier 3 antibody small ubiquitin-related modifier 3 antibody SMT3 homolog 1 antibody SMT3 homolog 2 antibody SMT3 suppressor of mif two 3 homolog 1 antibody SMT3 suppressor of mif two 3 homolog 2 (S. cerevisiae) antibody SMT3 suppressor of mif two 3 homolog 2 antibody SMT3 suppressor of mif two 3 homolog 3 (S. cerevisiae) antibody SMT3 suppressor of mif two 3 homolog 3 antibody SMT3A antibody SMT3B antibody SMT3H1 antibody SMT3H2 antibody Sumo2 antibody Sumo3 antibody Ubiquitin like protein SMT3A antibody Ubiquitin like protein SMT3B antibody |
| Product description | The small ubiquitin-related modifier (SUMO) proteins, which include SUMO-1, SUMO-2 and SUMO-3, belong to the ubiquitin-like protein family. Like ubiquitin, the SUMO proteins are synthesized as precursor proteins that undergo processing before conjugation to target proteins. Also, both utilize the E1, E2, and E3 cascade enzymes for conjugation. However, SUMO and ubiquitin differ with respect to targeting. Ubiquitination predominantly targets proteins for degradation, whereas sumoylation targets proteins to a variety of cellular processing, including nuclear transport, transcriptional regulation, apoptosis and protein stability. The unconjugated SUMO-1, SUMO-2 and SUMO-3 proteins localize to the nuclear membrane, nuclear bodies and cytoplasm, respectively. SUMO-1 utilizes Ubc9 for conjugation to several target proteins, which include IkBa, MDM2, p53, PML and Ran GAP1. SUMO-2 and SUMO-3 contribute to a greater percentage of protein modification than does SUMO-1, and unlike SUMO-1, they can form polymeric chains. In addition, SUMO-3 regulates b-Amyloid generation and may be critical in the onset or progression of Alzheimer's disease. |
| Immunogen | recombinant protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Recombinant rabbit |
| Tested Applications | WBICC/IFIHC |
| WB:1:1,000 ICC:1:50-1:200 IHC:1:50-1:200 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Alternative Names | HSMT3 antibody MGC117191 antibody OTTHUMP00000115275 antibody OTTHUMP00000115276 antibody OTTHUMP00000115277 antibody Sentrin 2 antibody Small ubiquitin like modifier 2 antibody Small ubiquitin related modifier 2 antibody small ubiquitin-like modifier 3 antibody small ubiquitin-related modifier 3 antibody SMT3 homolog 1 antibody SMT3 homolog 2 antibody SMT3 suppressor of mif two 3 homolog 1 antibody SMT3 suppressor of mif two 3 homolog 2 (S. cerevisiae) antibody SMT3 suppressor of mif two 3 homolog 2 antibody SMT3 suppressor of mif two 3 homolog 3 (S. cerevisiae) antibody SMT3 suppressor of mif two 3 homolog 3 antibody SMT3A antibody SMT3B antibody SMT3H1 antibody SMT3H2 antibody Sumo2 antibody Sumo3 antibody Ubiquitin like protein SMT3A antibody Ubiquitin like protein SMT3B antibody |
|---|---|
| Molecular Weight(MW) | 12 kDa |
| Cellular Localization | Cytoplasm, Nucleus. |
Application
Fig1: Western blot analysis of Sumo 2+3 on SW480 cells lysates using anti- Sumo 2+3 antibody at 1/1,000 dilution.
Application
Fig2: ICC staining Sumo 2+3 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig3: ICC staining Sumo 2+3 in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig4: ICC staining Sumo 2+3 in RH-35 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig5: Immunohistochemical analysis of paraffin-embedded mouse lung tissue using anti- Sumo 2+3 antibody. Counter stained with hematoxylin.
Application
Fig6: Immunohistochemical analysis of paraffin-embedded mouse skin tissue using anti- Sumo 2+3 antibody. Counter stained with hematoxylin.
Application
Fig7: Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti- Sumo 2+3 antibody. Counter stained with hematoxylin.
Application
Fig8: Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using anti- Sumo 2+3 antibody. Counter stained with hematoxylin.| Positive Control | SW480, A549, Hela, RH-35, human kidney tissue, mouse kidney tissue, mouse lung tissue, mouse kidney tissue, mouse skin tissue. |
|---|---|
| Application Notes | WB:1:1,000 ICC:1:50-1:200 IHC:1:50-1:200 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
