Application
Fig1: Western blot analysis of A-RAF on Hela cells lysates using anti-A-RAF antibody at 1/1,000 dilution.Application
Fig2: ICC staining A-RAF in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig3: ICC staining A-RAF in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig4: ICC staining A-RAF in NIH/3T3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig5: Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-A-RAF antibody. Counter stained with hematoxylin.Application
Fig6: Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-A-RAF antibody. Counter stained with hematoxylin.Application
Fig7: Flow cytometric analysis of Hela cells with A-RAF antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.| Product Name | A-RAF [JJ08-68] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | A raf 1 antibody A Raf proto oncogene serine/threonine protein kinase antibody ARAF 1 antibody Araf antibody ARaf proto oncogene serine/threonine protein kinase antibody ARAF_HUMAN antibody ARAF1 antibody Oncogene Araf1 antibody Oncogene PKS2 antibody PKS 2 antibody PKS antibody PKS2 antibody Proto oncogene Pks antibody Proto-oncogene A-Raf antibody Proto-oncogene A-Raf-1 antibody Proto-oncogene Pks antibody RAFA 1 antibody RAFA1 antibody Ras binding protein DA Raf antibody Serine/threonine-protein kinase A-Raf antibody v raf murine sarcoma 3611 viral oncogene homolog antibody v raf murine sarcoma 3611 viral oncogene homolog 1 antibody v raf oncogene homolog 1 (murine sarcoma 3611 virus) antibody |
| Product description | Several serine/threonine protein kinases have been implicated as intermediates in signal transduction pathways. These include ERK/MAP kinases, ribosomal S6 kinase (Rsk) and Raf-1. Raf-1 is a cytoplasmic protein with intrinsic serine/ threonine activity. It is broadly expressed in nearly all cell lines tested to date and is the cellular homolog of v-Raf, the product of the transforming gene of the 3611 strain of murine sarcoma virus. The unregulated kinase activity of the v-Raf protein has been associated with transformation and mitogenesis while the activity of Raf-1 is normally suppressed by a regulatory N-terminal domain. A-Raf, a second member of the Raf gene family of serine/threonine protein kinases, exhibits substantial homology to Raf-1 within the kinase domain of the two molecules, but less homology elsewhere. Expression of A-Raf is found at highest levels in urogenital tissues and kidney and at lowest level in brain tissue. |
| Immunogen | recombinant protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Recombinant rabbit |
| Tested Applications | WBICC/IFIHCFC |
| WB:1:1,000-1:2,000 ICC:1:50-1:200 IHC:1:50-1:200 FC:1:50-1:100 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Alternative Names | A raf 1 antibody A Raf proto oncogene serine/threonine protein kinase antibody ARAF 1 antibody Araf antibody ARaf proto oncogene serine/threonine protein kinase antibody ARAF_HUMAN antibody ARAF1 antibody Oncogene Araf1 antibody Oncogene PKS2 antibody PKS 2 antibody PKS antibody PKS2 antibody Proto oncogene Pks antibody Proto-oncogene A-Raf antibody Proto-oncogene A-Raf-1 antibody Proto-oncogene Pks antibody RAFA 1 antibody RAFA1 antibody Ras binding protein DA Raf antibody Serine/threonine-protein kinase A-Raf antibody v raf murine sarcoma 3611 viral oncogene homolog antibody v raf murine sarcoma 3611 viral oncogene homolog 1 antibody v raf oncogene homolog 1 (murine sarcoma 3611 virus) antibody |
|---|---|
| Molecular Weight(MW) | 68 kDa |
| Cellular Localization | Cytosol. |
| SwissProt ID | P10398 |
|---|
Application
Fig1: Western blot analysis of A-RAF on Hela cells lysates using anti-A-RAF antibody at 1/1,000 dilution.
Application
Fig2: ICC staining A-RAF in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig3: ICC staining A-RAF in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig4: ICC staining A-RAF in NIH/3T3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig5: Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-A-RAF antibody. Counter stained with hematoxylin.
Application
Fig6: Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-A-RAF antibody. Counter stained with hematoxylin.
Application
Fig7: Flow cytometric analysis of Hela cells with A-RAF antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.| Positive Control | A431, NIH/3T3, Hela, human breast carcinoma tissue, human kidney tissue. |
|---|---|
| Application Notes | WB:1:1,000-1:2,000 ICC:1:50-1:200 IHC:1:50-1:200 FC:1:50-1:100 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
