Application
Fig1: ICC staining Cdk7 in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig2: ICC staining Cdk7 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig3: ICC staining Cdk7 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig4: ICC staining Cdk7 in 293 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig5: Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-Cdk7 antibody. Counter stained with hematoxylin.Application
Fig6: Immunohistochemical analysis of paraffin-embedded human lung cancer tissue using anti-Cdk7 antibody. Counter stained with hematoxylin.Application
Fig7: Flow cytometric analysis of A431 cells with Cdk7 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.| Product Name | Cdk7 [JJ203-01] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | 39 kDa protein kinase antibody CAK antibody CAK1 antibody Cdk activating kinase antibody CDK-activating kinase 1 antibody CDK-activating kinase antibody cdk7 antibody CDK7_HUMAN antibody CDKN7 antibody Cell division protein kinase 7 antibody Cyclin dependent kinase 7 antibody cyclin-dependent kinase 7 (MO15 homolog, Xenopus laevis, cdk-activating kinase) antibody Cyclin-dependent kinase 7 antibody HCAK antibody Homolog of Xenopus MO15 Cdk activating kinase antibody Kinase subunit of CAK antibody MO15 antibody MO15, Xenopus, homolog of antibody P39 Mo15 antibody p39MO15 antibody Serine threonine kinase Stk1 antibody Serine/threonine protein kinase 1 antibody Serine/threonine protein kinase MO15 antibody STK1 antibody TFIIH basal transcription factor complex kinase subunit antibody |
| Product description | Progression through the cell cycle requires activation of a series of enzymes designated cyclin dependent kinases (Cdks). The monomeric catalytic subunit Cdk2, a critical enzyme for initiation of cell cycle progression, is completely inactive. Partial activation is achieved by the binding of regulatory cyclins such as cyclin D1, while full activation requires additional phosphorylation at Thr 160. The enzyme responsible for the phosphorylation of Cdk2 on Thr 160 and also of Cdc2 p34 on Thr 161, designated Cdk-activating kinase (CAK), has been partially purified and shown to be comprised of a catalytic subunit and a regulatory subunit. The catalytic subunit, designated Cdk7, has been identified as the mammalian homolog of MO15, a protein kinase demonstrated in starfish and Xenopus. The regulatory subunit is a novel cyclin (cyclin H) and is required for activation of Cdk7. Like other Cdks, Cdk7 contains a conserved threonine residue required for full activity; mutation of this residue severely reduces CAK activity. |
| Immunogen | recombinant protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Recombinant rabbit |
| Tested Applications | WBICC/IFIHCFC |
| WB:1:1,000 ICC:1:100-1:500 IHC:1:50-1:200 FC:1:50-1:100 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Alternative Names | 39 kDa protein kinase antibody
CAK antibody
CAK1 antibody
Cdk activating kinase antibody
CDK-activating kinase 1 antibody
CDK-activating kinase antibody
cdk7 antibody
CDK7_HUMAN antibody
CDKN7 antibody
Cell division protein kinase 7 antibody
Cyclin dependent kinase 7 antibody
cyclin-dependent kinase 7 (MO15 homolog Xenopus laevis cdk-activating kinase) antibody Cyclin-dependent kinase 7 antibody HCAK antibody Homolog of Xenopus MO15 Cdk activating kinase antibody Kinase subunit of CAK antibody MO15 antibody MO15 Xenopus homolog of antibody P39 Mo15 antibody p39MO15 antibody Serine threonine kinase Stk1 antibody Serine/threonine protein kinase 1 antibody Serine/threonine protein kinase MO15 antibody STK1 antibody TFIIH basal transcription factor complex kinase subunit antibody |
|---|---|
| Molecular Weight(MW) | 39 kDa |
| Cellular Localization | Nucleus, Cytoplasm. |
| SwissProt ID | P50613 |
|---|
Application
Fig1: ICC staining Cdk7 in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig2: ICC staining Cdk7 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig3: ICC staining Cdk7 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig4: ICC staining Cdk7 in 293 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig5: Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-Cdk7 antibody. Counter stained with hematoxylin.
Application
Fig6: Immunohistochemical analysis of paraffin-embedded human lung cancer tissue using anti-Cdk7 antibody. Counter stained with hematoxylin.
Application
Fig7: Flow cytometric analysis of A431 cells with Cdk7 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.| Positive Control | A431, 293, Hela, HepG2, human lung cancer tissue, human breast carcinoma tissue. |
|---|---|
| Application Notes | WB:1:1,000 ICC:1:100-1:500 IHC:1:50-1:200 FC:1:50-1:100 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
