Application
Fig1: ICC staining ATF1 in HUVEC cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig2: ICC staining ATF1 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig3: Immunohistochemical analysis of paraffin-embedded human lung cancer tissue using anti-ATF1 antibody. Counter stained with hematoxylin.Application
Fig4: Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-ATF1 antibody. Counter stained with hematoxylin.Application
Fig5: Flow cytometric analysis of Hela cells with ATF1 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.| Product Name | ATF1 [JJ207-04] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Activating transcription factor 1 antibody ATF 1 antibody atf1 antibody ATF1 EWS fusion gene antibody ATF1 FUS fusion gene antibody ATF1_HUMAN antibody cAMP dependent transcription factor 1 antibody cAMP-dependent transcription factor ATF-1 antibody Cyclic AMP dependent transcription factor ATF 1 antibody Cyclic AMP dependent transcription factor ATF1 antibody Cyclic AMP-dependent transcription factor ATF-1 antibody EWS AFT1 antibody FUS ATF 1 antibody FUS/ATF 1 antibody Protein TREB36 antibody RNA binding protein activating transcription factor 1 fusion protein antibody TREB 36 antibody TREB36 antibody TREB36 protein antibody |
| Product description | Eukaryotic gene transcription is regulated by sequence-specific transcription factors that bind modular cis acting promoter and enhancer elements. The ATF/ CREB transcription factor family binds the palindromic cAMP response element (CRE) octanucleotide TGACGTCA. The ATF/CREB family includes CREB-1, CREB-2 (also designated ATF-4), ATF-1, ATF-2 and ATF-3. This family of proteins contain highly divergent N-terminal domains, but share a C-terminal leucine zipper for dimerization and DNA binding. ATF-1 is shown to play a key role in the induction of NOX1. ATF-1 binds the cAMP response element (CRE) and mediates PKA-induced stimulation of CRE-reporter genes. ATF-2 forms homodimers and heterodimers with c-Jun to initiate CRE-dependent transcription. Phosphorylation of ATF-2 at Thr 69 and Thr 71 by stress-activated kinases is necessary for transcriptional activation. Myc also induces phosphorylation of ATF-2 at Thr 69 and Thr 71 to prolong the half-life of ATF-2. ATF-2 functions as a histone acetyltransferase (HAT) and acetylates Histones H2B and H4 specifically in vitro. |
| Immunogen | recombinant protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Recombinant rabbit |
| Tested Applications | WBICC/IFIHCFC |
| WB:1 500-1:1,000ICC:1:50-1:200 IHC:1:50-1:200 FC:1:50-1:100 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Alternative Names | Activating transcription factor 1 antibody ATF 1 antibody atf1 antibody ATF1 EWS fusion gene antibody ATF1 FUS fusion gene antibody ATF1_HUMAN antibody cAMP dependent transcription factor 1 antibody cAMP-dependent transcription factor ATF-1 antibody Cyclic AMP dependent transcription factor ATF 1 antibody Cyclic AMP dependent transcription factor ATF1 antibody Cyclic AMP-dependent transcription factor ATF-1 antibody EWS AFT1 antibody FUS ATF 1 antibody FUS/ATF 1 antibody Protein TREB36 antibody RNA binding protein activating transcription factor 1 fusion protein antibody TREB 36 antibody TREB36 antibody TREB36 protein antibody |
|---|---|
| Molecular Weight(MW) | 29 kDa |
| Cellular Localization | Nucleus. |
| SwissProt ID | P18846 |
|---|
Application
Fig1: ICC staining ATF1 in HUVEC cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig2: ICC staining ATF1 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig3: Immunohistochemical analysis of paraffin-embedded human lung cancer tissue using anti-ATF1 antibody. Counter stained with hematoxylin.
Application
Fig4: Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-ATF1 antibody. Counter stained with hematoxylin.
Application
Fig5: Flow cytometric analysis of Hela cells with ATF1 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.| Positive Control | HUVEC, Hela, human lung cancer tissue, human breast carcinoma tissue. |
|---|---|
| Application Notes | WB:1 500-1:1,000ICC:1:50-1:200 IHC:1:50-1:200 FC:1:50-1:100 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
