Application
Fig1: ICC staining ATF7 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig2: ICC staining ATF7 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig3: Immunohistochemical analysis of paraffin-embedded mouse lung tissue using anti-ATF7 antibody. Counter stained with hematoxylin.Application
Fig4: Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue using anti-ATF7 antibody. Counter stained with hematoxylin.Application
Fig5: Immunohistochemical analysis of paraffin-embedded mouse heart tissue using anti-ATF7 antibody. Counter stained with hematoxylin.Application
Fig6: Flow cytometric analysis of Jurkat cells with ATF7 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody| Product Name | ATF7 [JJ2-072] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | 1110012F10Rik antibody 9430065F09Rik antibody Activating transcription factor 7 antibody AI549878 antibody ATF7 antibody Atf7 protein antibody ATF7_HUMAN antibody ATFA antibody C130020M04Rik antibody cAMP-dependent transcription factor ATF-7 antibody Cyclic AMP-dependent transcription factor ATF 7 antibody Cyclic AMP-dependent transcription factor ATF-7 antibody MGC31554 antibody MGC57182 antibody Transcription factor ATF A antibody Transcription factor ATF-A antibody |
| Product description | Plays important functions in early cell signaling. Binds the cAMP response element (CRE) (consensus: 5'-GTGACGT[AG][AG]-3'), a sequence present in many viral and cellular promoters. Activator of the NF-ELAM1/delta-A site of the E-selectin promoter. Has no intrinsic transcriptional activity, but activates transcription on formation of JUN or FOS heterodimers. Also can bind TRE promoter sequences when heterodimerized with members of the JUN family.Isoform 4/ATF-A0 acts as a dominant repressor of the E-selectin/NF-ELAM1/delta-A promoter.Isoform 5/ATF-4 acts as a negative regulator, inhibiting both ATF2 and ATF7 transcriptional activities. It may exert these effects by sequestrating in the cytoplasm the Thr-53 phosphorylating kinase, preventing activation. |
| Immunogen | recombinant protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Recombinant rabbit |
| Tested Applications | WBICC/IFIHCFC |
| WB:1:500-1:1,000 ICC:1:50-1:200 IHC:1:50-1:200 FC:1:50-1:100 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Alternative Names | 1110012F10Rik antibody 9430065F09Rik antibody Activating transcription factor 7 antibody AI549878 antibody ATF7 antibody Atf7 protein antibody ATF7_HUMAN antibody ATFA antibody C130020M04Rik antibody cAMP-dependent transcription factor ATF-7 antibody Cyclic AMP-dependent transcription factor ATF 7 antibody Cyclic AMP-dependent transcription factor ATF-7 antibody MGC31554 antibody MGC57182 antibody Transcription factor ATF A antibody Transcription factor ATF-A antibody |
|---|---|
| Molecular Weight(MW) | 53 kDa |
| Cellular Localization | Nucleus, Cytoplasm. |
Application
Fig1: ICC staining ATF7 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig2: ICC staining ATF7 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig3: Immunohistochemical analysis of paraffin-embedded mouse lung tissue using anti-ATF7 antibody. Counter stained with hematoxylin.
Application
Fig4: Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue using anti-ATF7 antibody. Counter stained with hematoxylin.
Application
Fig5: Immunohistochemical analysis of paraffin-embedded mouse heart tissue using anti-ATF7 antibody. Counter stained with hematoxylin.
Application
Fig6: Flow cytometric analysis of Jurkat cells with ATF7 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody| Positive Control | Hela, HepG2, mouse lung tissue, mouse skeletal muscle tissue, mouse heart tissue. |
|---|---|
| Application Notes | WB:1:500-1:1,000 ICC:1:50-1:200 IHC:1:50-1:200 FC:1:50-1:100 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
