Application
Fig1: ICC staining Phospho-AMPK alpha 2(S345) in 293 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig2: ICC staining Phospho-AMPK alpha 2(S345) in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig3: Immunohistochemical analysis of paraffin-embedded human pancreas tissue using anti-Phospho-AMPK alpha 2(S345) antibody. Counter stained with hematoxylin.Application
Fig4: Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue using anti-Phospho-AMPK alpha 2(S345) antibody. Counter stained with hematoxylin.| Product Name | Phospho-AMPK alpha 2(S345) [JJ08-19] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | 5'-AMP-activated protein kinase catalytic subunit alpha-2 antibody AAPK2_HUMAN antibody ACACA kinase antibody Acetyl-CoA carboxylase kinase antibody AMPK alpha 2 chain antibody AMPK subunit alpha-2 antibody AMPK2 antibody AMPKa2 antibody AMPKalpha2 antibody HMGCR kinase antibody Hydroxymethylglutaryl-CoA reductase kinase antibody PRKAA antibody PRKAA2 antibody Protein kinase AMP activated alpha 2 catalytic subunit antibody Protein kinase AMP activated catalytic subunit alpha 2 antibody |
| Product description | Five-prime-AMP-activated protein kinase, known as AMPK, is a heterotrimeric complex that comprises of a catalytic α subunit, and regulatory β and γ. AMPK protects cells from stresses that cause ATP depletion by switching off ATP-consuming biosynthetic pathways. AMPK is activated by high AMP and low ATP via a mechanism involving allosteric regulation, promotion of phosphorylation by an upstream protein kinase known as AMPK kinase (AMPKK), and inhibition of dephosphorylation. Activated AMPK can phosphorylate and regulate in vivo hydroxymethylglutaryl-CoA reductase and acetyl-CoA carboxylase, which are key regulatory enzymes of sterol synthesis and fatty acid synthesis, respectively. The human AMPKα1 gene maps to chromosome 5p12 and encodes a 548 amino acid protein. The major regulatory site phosphorylated by AMPKK on AMPKα has been identified as Thr 172 within the activation loop between the DFG and APE motifs of the alpha-subunits. |
| Immunogen | Synthetic phospho-peptide corresponding to residues surrounding Ser345 of human AMPK alpha 2. |
| Modification | p-S345 |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Recombinant rabbit |
| Tested Applications | WBICC/IFIHC |
| WB:1:500-1:1,000 ICC:1:50-1:200 IHC:1:50-1:100 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Alternative Names | 5'-AMP-activated protein kinase catalytic subunit alpha-2 antibody AAPK2_HUMAN antibody ACACA kinase antibody Acetyl-CoA carboxylase kinase antibody AMPK alpha 2 chain antibody AMPK subunit alpha-2 antibody AMPK2 antibody AMPKa2 antibody AMPKalpha2 antibody HMGCR kinase antibody Hydroxymethylglutaryl-CoA reductase kinase antibody PRKAA antibody PRKAA2 antibody Protein kinase AMP activated alpha 2 catalytic subunit antibody Protein kinase AMP activated catalytic subunit alpha 2 antibody |
|---|---|
| Molecular Weight(MW) | 62 kDa |
| Cellular Localization | Cytoplasm, Nucleus. |
Application
Fig1: ICC staining Phospho-AMPK alpha 2(S345) in 293 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig2: ICC staining Phospho-AMPK alpha 2(S345) in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig3: Immunohistochemical analysis of paraffin-embedded human pancreas tissue using anti-Phospho-AMPK alpha 2(S345) antibody. Counter stained with hematoxylin.
Application
Fig4: Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue using anti-Phospho-AMPK alpha 2(S345) antibody. Counter stained with hematoxylin.| Positive Control | 293, Hela, human pancreas tissue, mouse pancreas tissue. |
|---|---|
| Application Notes | WB:1:500-1:1,000 ICC:1:50-1:200 IHC:1:50-1:100 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
