Application
Fig1: Western blot analysis of MEK3+MEK6 on different lysates using anti-MEK3+MEK6 antibody at 1/1,000 dilution. Positive control: Lane 1: Hela Lane 2: JurkatApplication
Fig2: ICC staining MEK3+MEK6 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig3: ICC staining MEK3+MEK6 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig4: ICC staining MEK3+MEK6 in NIH/3T3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig5: Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-MEK3+MEK6 antibody. Counter stained with hematoxylin.Application
Fig6: Flow cytometric analysis of Hela cells with MEK3+MEK6 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody| Product Name | MEK3+MEK6 [JJ087-09] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Dual specificity mitogen activated protein kinase kinase 6 antibody Dual specificity mitogen-activated protein kinase kinase 3 antibody MAP kinase kinase 3 antibody MAP kinase kinase 6 antibody map2k3 antibody MAP2K6 antibody MAPK/ERK kinase 3 antibody MAPK/ERK kinase 6 antibody MAPKK 3 antibody MAPKK 6 antibody MAPKK3 antibody MAPKK6 antibody MEK 3 antibody MEK 6 antibody Mitogen activated protein kinase kinase 3 antibody Mitogen activated protein kinase kinase 6 antibody MKK3 antibody MKK6 antibody MP2K3_HUMAN antibody PRKMK3 antibody PRKMK6 antibody Protein kinase, mitogen activated, kinase 6 (MAP kinase kinase 6) antibody SAPK kinase 2 antibody SAPKK 3 antibody SAPKK-2 antibody SAPKK2 antibody SAPKK3 antibody Stress activated protein kinase kinase 3 antibody Stress-activated protein kinase kinase 2 antibody |
| Product description | A family of protein kinases located upstream of the MAP kinases and responsible for their activation has been identified. The prototype member of this family, designated MAP kinase kinase, or MEK-1, specifically phosphorylates the MAP kinase regulatory threonine and tyrosine residues present in the Thr-Glu-Tyr motif of ERK. A second MEK family member, MEK-2, resembles MEK-1 in its substrate specificity. MEK-3 (or MKK-3) functions to activate p38 MAP kinase, and MEK-4 (also called SEK1 or MKK-4) activates both p38 and JNK MAP kinases. MEK-5 appears to specifically phosphorylate ERK5, whereas MEK-6 phosphorylates p38 and p38b. MEK-7 (or MKK-7) phosphorylates and activates the JNK signal transduction pathway. |
| Immunogen | recombinant protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Recombinant rabbit |
| Tested Applications | WBICC/IFIHCIPFC |
| WB:1:1,000-1:2,000 ICC:1:50-1:200 IHC:1:50-1:200 FC:1:50-1:100 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Alternative Names | Dual specificity mitogen activated protein kinase kinase 6 antibody
Dual specificity mitogen-activated protein kinase kinase 3 antibody
MAP kinase kinase 3 antibody
MAP kinase kinase 6 antibody
map2k3 antibody
MAP2K6 antibody
MAPK/ERK kinase 3 antibody
MAPK/ERK kinase 6 antibody
MAPKK 3 antibody
MAPKK 6 antibody
MAPKK3 antibody
MAPKK6 antibody
MEK 3 antibody
MEK 6 antibody
Mitogen activated protein kinase kinase 3 antibody
Mitogen activated protein kinase kinase 6 antibody
MKK3 antibody
MKK6 antibody
MP2K3_HUMAN antibody
PRKMK3 antibody
PRKMK6 antibody
Protein kinase mitogen activated kinase 6 (MAP kinase kinase 6) antibody SAPK kinase 2 antibody SAPKK 3 antibody SAPKK-2 antibody SAPKK2 antibody SAPKK3 antibody Stress activated protein kinase kinase 3 antibody Stress-activated protein kinase kinase 2 antibody |
|---|---|
| Molecular Weight(MW) | 39/37 kDa |
| Cellular Localization | Cytoplasm, Nucleus. |
Application
Fig1: Western blot analysis of MEK3+MEK6 on different lysates using anti-MEK3+MEK6 antibody at 1/1,000 dilution. Positive control: Lane 1: Hela Lane 2: Jurkat
Application
Fig2: ICC staining MEK3+MEK6 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig3: ICC staining MEK3+MEK6 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig4: ICC staining MEK3+MEK6 in NIH/3T3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig5: Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-MEK3+MEK6 antibody. Counter stained with hematoxylin.
Application
Fig6: Flow cytometric analysis of Hela cells with MEK3+MEK6 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody| Positive Control | Jurkat, NIH/3T3, Hela, HepG2, human spleen tissue. |
|---|---|
| Application Notes | WB:1:1,000-1:2,000 ICC:1:50-1:200 IHC:1:50-1:200 FC:1:50-1:100 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
