Application
Fig1: Western blot analysis of Integrin alpha 5 on Hela cells lysates using anti-Integrin alpha 5 antibody at 1/1,000 dilution.Application
Fig2: ICC staining Integrin alpha 5 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig3: ICC staining Integrin alpha 5 in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig4: Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-Integrin alpha 5 antibody. Counter stained with hematoxylin.Application
Fig5: Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-Integrin alpha 5 antibody. Counter stained with hematoxylin.Application
Fig6: Immunohistochemical analysis of paraffin-embedded human uterus tissue using anti-Integrin alpha 5 antibody. Counter stained with hematoxylin.Application
Fig7: Immunohistochemical analysis of paraffin-embedded mouse uterus tissue using anti-Integrin alpha 5 antibody. Counter stained with hematoxylin.Application
Fig8: Flow cytometric analysis of Jurkat cells with Integrin alpha 5 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.| Product Name | Integrin alpha 5 [JJ08-94] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | CD49 antigen-like family member E antibody CD49e antibody Fibronectin receptor subunit alpha antibody Fibronectin receptor, alpha subunit antibody FNRA antibody Integrin alpha 5 (fibronectin receptor alpha) antibody Integrin alpha-5 antibody Integrin alpha-5 light chain antibody Integrin alpha-F antibody Integrin, alpha 5 (fibronectin receptor, alpha polypeptide) antibody ITA5_HUMAN antibody Itga5 antibody Very late activation protein 5, alpha subunit antibody VLA-5 antibody VLA5 antibody VLA5A antibody |
| Product description | Integrins are heterodimers composed of noncovalently associated transmembrane α and β subunits. The 16 α and 8 β subunits heterodimerize to produce more than 20 different receptors. Most integrin receptors bind ligands that are components of the extracellular matrix, including Fibronectin, Collagen and Vitronectin. Certain integrins can also bind to soluble ligands such as Fibrinogen, or to counterreceptors on adjacent cells such as the intracellular adhesion molecules (ICAMs), leading to aggregation of cells. Ligands serve to cross-link or cluster integrins by binding to adjacent integrin receptors; both receptor clustering and ligand occupancy are necessary for the activation of integrin-mediated responses. In addition to mediating cell adhesion and cytoskeletal organization, integrins function as signaling receptors. Signals transduced by integrins play a role in many biological processes, including cell growth, differentiation, migration and apoptosis. |
| Immunogen | recombinant protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Recombinant rabbit |
| Tested Applications | WBICC/IFIHCIPFC |
| WB:1:1,000 ICC:1:50-1:200 IHC:1:50-1:200 FC:1:50-1:100 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Alternative Names | CD49 antigen-like family member E antibody
CD49e antibody
Fibronectin receptor subunit alpha antibody
Fibronectin receptor alpha subunit antibody FNRA antibody Integrin alpha 5 (fibronectin receptor alpha) antibody Integrin alpha-5 antibody Integrin alpha-5 light chain antibody Integrin alpha-F antibody Integrin alpha 5 (fibronectin receptor alpha polypeptide) antibody ITA5_HUMAN antibody Itga5 antibody Very late activation protein 5 alpha subunit antibody VLA-5 antibody VLA5 antibody VLA5A antibody |
|---|---|
| Molecular Weight(MW) | 150 kDa |
| Cellular Localization | Membrane, Cell junction, Cell surface. |
Application
Fig1: Western blot analysis of Integrin alpha 5 on Hela cells lysates using anti-Integrin alpha 5 antibody at 1/1,000 dilution.
Application
Fig2: ICC staining Integrin alpha 5 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig3: ICC staining Integrin alpha 5 in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig4: Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-Integrin alpha 5 antibody. Counter stained with hematoxylin.
Application
Fig5: Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-Integrin alpha 5 antibody. Counter stained with hematoxylin.
Application
Fig6: Immunohistochemical analysis of paraffin-embedded human uterus tissue using anti-Integrin alpha 5 antibody. Counter stained with hematoxylin.
Application
Fig7: Immunohistochemical analysis of paraffin-embedded mouse uterus tissue using anti-Integrin alpha 5 antibody. Counter stained with hematoxylin.
Application
Fig8: Flow cytometric analysis of Jurkat cells with Integrin alpha 5 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.| Positive Control | A549, Hela, human tonsil tissue, mouse uterus tissue, human kidney tissue, human uterus tissue. |
|---|---|
| Application Notes | WB:1:1,000 ICC:1:50-1:200 IHC:1:50-1:200 FC:1:50-1:100 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
