Application
Fig1: Western blot analysis of LC3B on different lysates using anti-LC3B antibody at 1/1,000 dilution. Positive control: Lane 1: Hela Lane 2: MCF-7| Product Name | LC3B [JJ090-6] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | ATG8F antibody Autophagy-related protein LC3 B antibody Autophagy-related ubiquitin-like modifier LC3 B antibody LC3B antibody LC3II antibody MAP1 light chain 3 like protein 2 antibody MAP1 light chain 3-like protein 2 antibody MAP1A/1BLC3 antibody MAP1A/MAP1B LC3 B antibody MAP1A/MAP1B light chain 3 B antibody MAP1ALC3 antibody MAP1LC3B a antibody Map1lc3b antibody Microtubule associated protein 1 light chain 3 beta antibody Microtubule-associated protein 1 light chain 3 beta antibody Microtubule-associated proteins 1A/1B light chain 3B antibody MLP3B_HUMAN antibody |
| Product description | Microtubule-associated proteins (MAPs) regulate microtubule stability and play critical roles in neuronal development and in maintaining the balance between neuronal plasticity and rigidity. MAP-light chain 3 beta (MAP-LC3β) and MAP-light chain 3 alpha (MAP-LC3α) are subunits of both MAP1A and MAP1B. MAP-LC3β, a homolog of Apg8p, is essential for autophagy and associated to the autophagosome membranes after processing. Two forms of LC3β, the cytosolic LC3-I and the membrane-bound LC3-II, are produced post-translationally. LC3-I is formed by the removal of the C-terminal 22 amino acids from newly synthesized LC3β, followed by the conversion of a fraction of LC3-I into LC3-II. LC3 enhances fibronectin mRNA translation in ductus arteriosus cells through association with 60S ribosomes and binding to an AU-rich element in the 3′ untranslated region of fibronectin mRNA. This facilitates sorting of fibronectin mRNA onto rough endoplasmic reticulum and translation. MAP LC3β may also be involved in formation of autophagosomal vacuoles. It is expressed primarily in heart, testis, brain and skeletal muscle. |
| Immunogen | recombinant protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Recombinant rabbit |
| Tested Applications | WBIP |
| WB:1:1,000-1:2,000 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Alternative Names | ATG8F antibody
Autophagy-related protein LC3 B antibody
Autophagy-related ubiquitin-like modifier LC3 B antibody LC3B antibody LC3II antibody MAP1 light chain 3 like protein 2 antibody MAP1 light chain 3-like protein 2 antibody MAP1A/1BLC3 antibody MAP1A/MAP1B LC3 B antibody MAP1A/MAP1B light chain 3 B antibody MAP1ALC3 antibody MAP1LC3B a antibody Map1lc3b antibody Microtubule associated protein 1 light chain 3 beta antibody Microtubule-associated protein 1 light chain 3 beta antibody Microtubule-associated proteins 1A/1B light chain 3B antibody MLP3B_HUMAN antibody |
|---|---|
| Molecular Weight(MW) | 14 kDa |
| Cellular Localization | Cytoplasm, Endomembrane system, Cytoplasmic vesicle. |
Application
Fig1: Western blot analysis of LC3B on different lysates using anti-LC3B antibody at 1/1,000 dilution. Positive control: Lane 1: Hela Lane 2: MCF-7| Positive Control | MCF-7, Hela. |
|---|---|
| Application Notes | WB:1:1,000-1:2,000 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
