Application
Fig1: Western blot analysis of CDC42 on different lysates using anti-CDC42 antibody at 1/1,000 dilution. Positive control: Lane 1: Hela Lane 2: Jurkat Lane 3: HepG2Application
Fig2: Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-CDC42 antibody. Counter stained with hematoxylin.Application
Fig3: Immunohistochemical analysis of paraffin-embedded human pancreas tissue using anti-CDC42 antibody. Counter stained with hematoxylin.Application
Fig4: Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue using anti-CDC42 antibody. Counter stained with hematoxylin.Application
Fig5: Flow cytometric analysis of Hela cells with CDC42 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.| Product Name | CDC42 [JJ086-04] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | CDC42 antibody CDC42_HUMAN antibody CDC42Hs antibody Cell division control protein 42 homolog antibody Cell division cycle 42 (GTP binding protein 25kDa) antibody Cell division cycle 42 antibody dJ224A6.1.1 (cell division cycle 42 (GTP-binding protein, 25kD)) antibody dJ224A6.1.2 (cell division cycle 42 (GTP-binding protein, 25kD)) antibody G25K antibody G25K GTP-binding protein antibody Growth regulating protein antibody GTP binding protein 25kDa antibody Small GTP binding protein CDC42 antibody TKS antibody |
| Product description | The superfamily of GTP-binding proteins, for which the Ras proteins are prototypes, has been implicated in regulation of diverse biological activities involving various aspects of cell growth and division. One mammalian member of the family, Cdc42, has an amino acid sequence that is similar to those of various members of the Ras superfamily proteins, including N-, K- and H-Ras, Rho proteins and the Rac proteins. On the basis of in vitro phosphorylation studies, it has been suggested that human Cdc42 may function in the signaling pathway of the EGF receptor or related growth factor receptor protein kinases. The Dbl oncogene has been shown to specifically catalyze dissociation of GDP from human Cdc42. |
| Immunogen | recombinant protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Recombinant rabbit |
| Tested Applications | WBIHCIPFC |
| WB:1:1,000 FC:1:50-1:100 IHC:1:50-1:200 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Alternative Names | CDC42 antibody
CDC42_HUMAN antibody
CDC42Hs antibody
Cell division control protein 42 homolog antibody
Cell division cycle 42 (GTP binding protein 25kDa) antibody
Cell division cycle 42 antibody
dJ224A6.1.1 (cell division cycle 42 (GTP-binding protein 25kD)) antibody dJ224A6.1.2 (cell division cycle 42 (GTP-binding protein 25kD)) antibody G25K antibody G25K GTP-binding protein antibody Growth regulating protein antibody GTP binding protein 25kDa antibody Small GTP binding protein CDC42 antibody TKS antibody |
|---|---|
| Molecular Weight(MW) | 21 kDa |
| Cellular Localization | Cell membrane, Cytoplasm, Midbody. |
Application
Fig1: Western blot analysis of CDC42 on different lysates using anti-CDC42 antibody at 1/1,000 dilution. Positive control: Lane 1: Hela Lane 2: Jurkat Lane 3: HepG2
Application
Fig2: Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-CDC42 antibody. Counter stained with hematoxylin.
Application
Fig3: Immunohistochemical analysis of paraffin-embedded human pancreas tissue using anti-CDC42 antibody. Counter stained with hematoxylin.
Application
Fig4: Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue using anti-CDC42 antibody. Counter stained with hematoxylin.
Application
Fig5: Flow cytometric analysis of Hela cells with CDC42 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.| Positive Control | Jurkat, Hela, HepG2, human breast carcinoma tissue, human pancreas tissue, mouse pancreas tissue. |
|---|---|
| Application Notes | WB:1:1,000 FC:1:50-1:100 IHC:1:50-1:200 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
