Application
Fig1: Western blot analysis of E2F1 on HepG2 cells lysates using anti-E2F1 antibody at 1/1,000 dilution.Application
Fig2: ICC staining E2F1 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig3: ICC staining E2F1 in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig4: ICC staining E2F1 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig5: Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue using anti-E2F1 antibody. Counter stained with hematoxylin.Application
Fig6: Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-E2F1 antibody. Counter stained with hematoxylin.Application
Fig7: Flow cytometric analysis of Hela cells with E2F1 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.| Product Name | E2F1 [JJ092-02] |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | The human retinoblastoma gene product appears to play an important role in the negative regulation of cell proliferation. Functional inactivation of Rb can be mediated either through mutation or as a consequence of interaction with DNA tumor virus-encoded proteins. Of all the Rb associations described to date, the identification of a complex between Rb and the transcription factor E2F most directly implicates Rb in regulation of cell proliferation. E2F was originally identified through its role in transcriptional activation of the adenovirus E2 promoter. Sequences homologous to the E2F binding site have been found upstream of a number of genes that encode proteins with putative functions in the G1 and S phases of the cell cycle. E2F-1 is a member of a broader family of transcription regulators including E2F-2, E2F-3, E2F-4, E2F-5, E2F-6 and E2F-7 each of which forms heterodimers with a second protein, DP-1, forming an "active" E2F transcriptional regulatory complex. |
| Immunogen | recombinant protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Recombinant rabbit |
| Tested Applications | WBICC/IFIHCIPFC |
| WB:1:1,000 ICC:1:100-1:500 IHC:1:50-1:200 FC:1:50-1:100 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Unpurified |
| Alternative Names | DmelCG6376 antibody Dmel_CG6376 antibody drosE2F1 antibody E(Sev-CycE)3A antibody E(var)3-93E antibody E2-promoter binding facto antibody E2F 1 antibody E2F transcription factor 1 antibody E2F-1 antibody E2f-PA antibody E2f-PB antibody E2f-PC antibody E2F1 antibody E2f1 E2F transcription factor 1 antibody E2F1_HUMAN antibody Evar(3)164 antibody KIAA4009 antibody l(3)07172 antibody l(3)j3B1 antibody l(3)j3C2 antibody l(3)rM729 antibody mKIAA4009 antibody OTTHUMP00000030661 antibody PBR3 antibody PRB binding protein E2F 1 antibody PRB-binding protein E2F-1 antibody RBAP 1 antibody RBAP-1 antibody RBAP1 antibody RBBP-3 antibody RBBP3 antibody RBP 3 antibody RBP3 antibody Retinoblastoma-associated protein 1 antibody Retinoblastoma-binding protein 3 antibody Transcription factor E2F1 antibody |
|---|---|
| Molecular Weight(MW) | 55 kDa |
| Cellular Localization | Nucleus. |
Application
Fig1: Western blot analysis of E2F1 on HepG2 cells lysates using anti-E2F1 antibody at 1/1,000 dilution.
Application
Fig2: ICC staining E2F1 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig3: ICC staining E2F1 in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig4: ICC staining E2F1 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig5: Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue using anti-E2F1 antibody. Counter stained with hematoxylin.
Application
Fig6: Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-E2F1 antibody. Counter stained with hematoxylin.
Application
Fig7: Flow cytometric analysis of Hela cells with E2F1 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.| Positive Control | MCF-7, Hela, HepG2, human breast carcinoma tissue, mouse pancreas tissue. |
|---|---|
| Application Notes | WB:1:1,000 ICC:1:100-1:500 IHC:1:50-1:200 FC:1:50-1:100 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
