Application
Fig1: Flow cytometric analysis of 293 cells with Occludin antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.| Product Name | Occludin [JJ091-08] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | BLCPMG antibody FLJ08163 antibody FLJ18079 antibody FLJ77961 antibody FLJ94056 antibody MGC34277 antibody Occludin antibody Ocln antibody OCLN_HUMAN antibody Tight junction protein occludin antibody |
| Product description | Occludin is an integral membrane protein closely associated with the tight junctions of epithelial and endothelial cells. Occludin is a tetraspan integral membrane protein in epithelial and endothelial tight junction (TJ) structures that can contain two extracellular loops. The protein exists in a variety of phosphorylated forms. Phosphorylation is involved in regulating both the localization and the function of occludin. Expression of occludin is up-regulated by poly-unsaturated fatty acids, increasing transendothelial cell resistance and reducing cellular permeability to large molecules. The level of occludin varies greatly depending on tissue; in brain tissue, occludin is highly expressed at cell-cell contact sites. Non-neural tissues show lower expression and discontinuous distribution. Up-regulation of epithelial occludin may play a role in enhancing paracellular permeability and be related to the damage to the tight junction. |
| Immunogen | recombinant protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Recombinant rabbit |
| Tested Applications | WBIPFC |
| WB:1:1,000 FC:1:50-1:100 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Alternative Names | BLCPMG antibody FLJ08163 antibody FLJ18079 antibody FLJ77961 antibody FLJ94056 antibody MGC34277 antibody Occludin antibody Ocln antibody OCLN_HUMAN antibody Tight junction protein occludin antibody |
|---|---|
| Molecular Weight(MW) | 59 kDa |
| Cellular Localization | Cell membrane, Cell junction. |
Application
Fig1: Flow cytometric analysis of 293 cells with Occludin antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.| Positive Control | 293 |
|---|---|
| Application Notes | WB:1:1,000 FC:1:50-1:100 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
