Application
Fig1: Western blot analysis of IGF2 on human placenta lysates using anti-IGF2 antibody at 1/1,000 dilution.Application
Fig2: ICC staining IGF2 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig3: ICC staining IGF2 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig4: ICC staining IGF2 in 293T cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig5: ICC staining IGF2 in 293 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig6: Immunohistochemical analysis of paraffin-embedded human liver cancer tissue using anti-IGF2 antibody. Counter stained with hematoxylin.Application
Fig7: Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-IGF2 antibody. Counter stained with hematoxylin.Application
Fig8: Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-IGF2 antibody. Counter stained with hematoxylin.| Product Name | IGF2 [JJ092-3] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | C11orf43 antibody IGF 2 antibody IGF II antibody IGF-II antibody IGF2 antibody IGF2_HUMAN antibody IGFII antibody INSIGF antibody insulin like growth factor 2 (somatomedin A) antibody Insulin like Growth Factor 2 antibody Insulin like growth factor II antibody Insulin like growth factor II precursor antibody Insulin like growth factor type 2 antibody pp9974 antibody Preptin antibody putative insulin like growth factor II associated protein antibody Somatomedin A antibody Somatomedin-A antibody |
| Product description | IGF-II (Insulin-like growth factor II; also multiplication-stimulating polypeptide/MSP and somatomedin-A) is a secreted 8 kDa polypeptide that belongs to the insulin family of peptide growth factors. It is part of a complex system of growth and metabolic-regulating proteins that is particularly important during development. It has been associated with nervous system proliferation and differentiation, myelination, adrenal cortical proliferation, and skeletal growth and differentiation. In human, IGF-II is primarily synthesized by the liver, and circulates at high levels in both fetus and adult. In rodent, however, IGF-II levels drop after the perinatal period, an effect attributed to the lack of a key gene promoter. This may indicate that postnatally, IGF-II has either a limited, or local effect only in rodent. |
| Immunogen | recombinant protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Recombinant rabbit |
| Tested Applications | WBICC/IFIHC |
| WB:1:1,000 ICC:1:100-1:500 IHC:1:50-1:200 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Alternative Names | C11orf43 antibody IGF 2 antibody IGF II antibody IGF-II antibody IGF2 antibody IGF2_HUMAN antibody IGFII antibody INSIGF antibody insulin like growth factor 2 (somatomedin A) antibody Insulin like Growth Factor 2 antibody Insulin like growth factor II antibody Insulin like growth factor II precursor antibody Insulin like growth factor type 2 antibody pp9974 antibody Preptin antibody putative insulin like growth factor II associated protein antibody Somatomedin A antibody Somatomedin-A antibody |
|---|---|
| Molecular Weight(MW) | 11 kDa |
| Cellular Localization | Secreted. |
| SwissProt ID | P01344 |
|---|
Application
Fig1: Western blot analysis of IGF2 on human placenta lysates using anti-IGF2 antibody at 1/1,000 dilution.
Application
Fig2: ICC staining IGF2 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig3: ICC staining IGF2 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig4: ICC staining IGF2 in 293T cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig5: ICC staining IGF2 in 293 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig6: Immunohistochemical analysis of paraffin-embedded human liver cancer tissue using anti-IGF2 antibody. Counter stained with hematoxylin.
Application
Fig7: Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-IGF2 antibody. Counter stained with hematoxylin.
Application
Fig8: Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-IGF2 antibody. Counter stained with hematoxylin.| Positive Control | 293, 293T, Hela, HepG2, human liver cancer tissue, human colon cancer tissue, human breast carcinoma tissue. |
|---|---|
| Application Notes | WB:1:1,000 ICC:1:100-1:500 IHC:1:50-1:200 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
