Application
Fig1: ICC staining Fatty Acid Synthase in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig2: ICC staining Fatty Acid Synthase in RH-35 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig3: ICC staining Fatty Acid Synthase in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig4: ICC staining Fatty Acid Synthase in SW480 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig5: Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-Fatty Acid Synthase antibody. Counter stained with hematoxylin.Application
Fig6: Immunohistochemical analysis of paraffin-embedded mouse colon tissue using anti-Fatty Acid Synthase antibody. Counter stained with hematoxylin.Application
Fig7: Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-Fatty Acid Synthase antibody. Counter stained with hematoxylin.Application
Fig8: Flow cytometric analysis of A549 cells with Fatty Acid Synthase antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.| Product Name | Fatty Acid Synthase [JJ0939] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | [Acyl-carrier-protein] S acetyltransferase antibody [Acyl-carrier-protein] S malonyltransferase antibody 3-hydroxypalmitoyl-[acyl-carrier-protein] dehydratase antibody 3-oxoacyl-[acyl-carrier-protein] reductase antibody 3-oxoacyl-[acyl-carrier-protein] synthase antibody Enoyl-[acyl-carrier-protein] reductase antibody FAS antibody FAS_HUMAN antibody FASN antibody Fatty acid synthase antibody MGC14367 antibody MGC15706 antibody OA 519 antibody Oleoyl-[acyl-carrier-protein] hydrolase antibody SDR27X1 antibody Short chain dehydrogenase/reductase family 27X member 1 antibody |
| Product description | Fatty acid biosynthesis is mediated by seven catalytic enzymes and an acyl carrier protein (ACP), to which various acyl intermediates are covalently attached. Fatty Acid Synthase (FAS) is the anabolic enzyme that contains the seven unique catalytic sites and mediates the conversion of acetyl-CoA and malonyl-CoA, in the presence of the cofactor NADPH, into long-chain saturated fatty acids, such as palmitate. Human Fatty Acid Synthase cDNA encodes a 2,504 amino acid protein. Catalytically active Fatty Acid Synthase is a homodimer. Human Fatty Acid Synthase mRNA is variably expressed with abundant levels present in brain, lung and liver. Fatty acid synthetic metabolism is abnormally elevated in tumor cells and may support cell growth or survival of malignant cancers. |
| Immunogen | recombinant protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Recombinant rabbit |
| Tested Applications | WBICC/IFIHCIPFC |
| WB:1:1,000 ICC:1:100-1:500 IHC:1:50-1:200 FC:1:50-1:100 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Alternative Names | [Acyl-carrier-protein] S acetyltransferase antibody [Acyl-carrier-protein] S malonyltransferase antibody 3-hydroxypalmitoyl-[acyl-carrier-protein] dehydratase antibody 3-oxoacyl-[acyl-carrier-protein] reductase antibody 3-oxoacyl-[acyl-carrier-protein] synthase antibody Enoyl-[acyl-carrier-protein] reductase antibody FAS antibody FAS_HUMAN antibody FASN antibody Fatty acid synthase antibody MGC14367 antibody MGC15706 antibody OA 519 antibody Oleoyl-[acyl-carrier-protein] hydrolase antibody SDR27X1 antibody Short chain dehydrogenase/reductase family 27X member 1 antibody |
|---|---|
| Molecular Weight(MW) | 273 kDa |
| Cellular Localization | Cytoplasm, Melanosome. |
Application
Fig1: ICC staining Fatty Acid Synthase in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig2: ICC staining Fatty Acid Synthase in RH-35 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig3: ICC staining Fatty Acid Synthase in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig4: ICC staining Fatty Acid Synthase in SW480 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig5: Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-Fatty Acid Synthase antibody. Counter stained with hematoxylin.
Application
Fig6: Immunohistochemical analysis of paraffin-embedded mouse colon tissue using anti-Fatty Acid Synthase antibody. Counter stained with hematoxylin.
Application
Fig7: Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-Fatty Acid Synthase antibody. Counter stained with hematoxylin.
Application
Fig8: Flow cytometric analysis of A549 cells with Fatty Acid Synthase antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.| Positive Control | A549, MCF-7, RH-35, SW480, human liver tissue, human breast carcinoma tissue, mouse colon tissue. |
|---|---|
| Application Notes | WB:1:1,000 ICC:1:100-1:500 IHC:1:50-1:200 FC:1:50-1:100 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
