Application
Fig1: ICC staining Fas in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig2: ICC staining Fas in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig3: Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-Fas antibody. Counter stained with hematoxylin.Application
Fig4: Immunohistochemical analysis of paraffin-embedded human liver cancer tissue using anti-Fas antibody. Counter stained with hematoxylin.Application
Fig5: Flow cytometric analysis of Raji cells with Fas antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.| Product Name | Fas(CD95) [JJ0942] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | ALPS 1A antibody ALPS1A antibody APO 1 antibody Apo 1 antigen antibody APO 1 cell surface antigen antibody Apo-1 antigen antibody APO1 antibody Apo1 antigen antibody APO1 cell surface antigen antibody Apoptosis antigen 1 antibody Apoptosis mediating surface antigen FAS antibody Apoptosis-mediating surface antigen FAS antibody APT 1 antibody APT1 antibody CD 95 antibody CD 95 antigen antibody CD95 antibody CD95 antigen antibody Delta Fas antibody Delta Fas/APO 1/CD95 antibody Delta Fas/APO1/CD95 antibody Fas (TNF receptor superfamily, member 6) antibody FAS 1 antibody FAS 827dupA antibody Fas AMA antibody Fas antibody FAS Antigen antibody Fas cell surface death receptor antibody FAS1 antibody FASLG receptor antibody FASTM antibody sFAS antibody Surface antigen APO1 antibody TNF receptor superfamily, member 6 antibody TNFRSF 6 antibody TNFRSF6 antibody TNR6_HUMAN antibody Tumor necrosis factor receptor superfamily member 6 antibody |
| Product description | Cytotoxic T lymphocyte (CTL)-mediated cytotoxicity constitutes an important component of specific effector mechanisms in immuno-surveillance against virus-infected or transformed cells. Two mechanisms appear to account for this activity, one of which is the perforin-based process. Independently, a FAS-based mechanism involves the transducing molecule FAS (also designated APO-1) and its ligand (FAS-L). The human FAS protein is a cell surface glycoprotein that belongs to a family of receptors that includes CD40, nerve growth factor receptors and tumor necrosis factor receptors. The FAS antigen is expressed on a broad range of lymphoid cell lines, certain of which undergo apoptosis in response to treatment with antibody to FAS. These findings strongly imply that targeted cell death is potentially mediated by the intercellular interactions of FAS with its ligand or effectors, and that FAS may be critically involved in CTL-mediated cytotoxicity. |
| Immunogen | recombinant protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Recombinant rabbit |
| Tested Applications | WBICC/IFIHCFC |
| WB:1:500-1:1,000 ICC:1:50-1:200 IHC:1:50-1:200 FC:1:50-1:100 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Alternative Names | ALPS 1A antibody
ALPS1A antibody
APO 1 antibody
Apo 1 antigen antibody
APO 1 cell surface antigen antibody
Apo-1 antigen antibody
APO1 antibody
Apo1 antigen antibody
APO1 cell surface antigen antibody
Apoptosis antigen 1 antibody
Apoptosis mediating surface antigen FAS antibody
Apoptosis-mediating surface antigen FAS antibody
APT 1 antibody
APT1 antibody
CD 95 antibody
CD 95 antigen antibody
CD95 antibody
CD95 antigen antibody
Delta Fas antibody
Delta Fas/APO 1/CD95 antibody
Delta Fas/APO1/CD95 antibody
Fas (TNF receptor superfamily member 6) antibody FAS 1 antibody FAS 827dupA antibody Fas AMA antibody Fas antibody FAS Antigen antibody Fas cell surface death receptor antibody FAS1 antibody FASLG receptor antibody FASTM antibody sFAS antibody Surface antigen APO1 antibody TNF receptor superfamily member 6 antibody TNFRSF 6 antibody TNFRSF6 antibody TNR6_HUMAN antibody Tumor necrosis factor receptor superfamily member 6 antibody |
|---|---|
| Molecular Weight(MW) | 45 kDa |
| Cellular Localization | Cell membrane, Secreted. |
| SwissProt ID | P25445 |
|---|
Application
Fig1: ICC staining Fas in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig2: ICC staining Fas in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig3: Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-Fas antibody. Counter stained with hematoxylin.
Application
Fig4: Immunohistochemical analysis of paraffin-embedded human liver cancer tissue using anti-Fas antibody. Counter stained with hematoxylin.
Application
Fig5: Flow cytometric analysis of Raji cells with Fas antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.| Positive Control | Hela, HepG2, human tonsil tissue, human liver cancer tissue. |
|---|---|
| Application Notes | WB:1:500-1:1,000 ICC:1:50-1:200 IHC:1:50-1:200 FC:1:50-1:100 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
