Application
Fig1: Western blot analysis of RPSA on different lysates using anti-RPSA antibody at 1/1,000 dilution. Positive control: Lane 1: K562 Lane 2: HepG2 Lane 3: A431Application
Fig2: ICC staining RPSA in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig3: ICC staining RPSA in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig4: ICC staining RPSA in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig5: ICC staining RPSA in RH-35 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig6: Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-RPSA antibody. Counter stained with hematoxylin.Application
Fig7: Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using anti-RPSA antibody. Counter stained with hematoxylin.Application
Fig8: Immunohistochemical analysis of paraffin-embedded human gastric carcinoma tissue using anti-RPSA antibody. Counter stained with hematoxylin.Application
Fig9: Flow cytometric analysis of MCF-7 cells with RPSA antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.| Product Name | 67kDa Laminin Receptor [JF0955] |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Laminin receptor (Laminin-R) has a heterodimeric structure similar to that of receptors for other extracellular matrix proteins such as Fibronectin and Vitronectin. Incorporation of Laminin-R into lysosomal membranes makes it possible for lysosomes to attach to surfaces coated with Laminin. This and other properties identify Laminin-R as a member of the integrin family of cell adhesion receptors. The Laminin-R precursor is a polypeptide whose expression is consistently upregulated in aggressive carcinoma. The precursor, which is also identified as p40 ribosome-associated protein, appears to be a multifunctional protein involved in the translational machinery. Laminin-R (also known as colon carcinoma laminin-binding protein) and is found at nine-fold higher levels in colon carcinoma than in adjacent normal colonic epithelium. Additionally, the level of the Laminin-R is higher in the lung cancer cell line than in the lung cell line. |
| Immunogen | recombinant protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Recombinant rabbit |
| Tested Applications | WB,ICC/IF,IHC,IP,FC |
| WB:1:1,000-1:2,000 ICC:1:100-1:500 IHC:1:50-1:200 FC:1:50-1:100 |
|
| Species Reactivity | Human,Mouse,Rat |
| Concentration | 1mg/ml |
| Purification | Unpurified |
| Alternative Names | 34/67 kDa laminin receptor antibody 37 kDa laminin receptor precursor antibody 37/67 kDa laminin receptor antibody 37LRP antibody 40S ribosomal protein SA antibody 67 kDa laminin receptor antibody 67LR antibody Colon carcinoma laminin binding protein antibody Colon carcinoma laminin-binding protein antibody LAMBR antibody Laminin receptor 1 antibody Laminin-binding protein precursor p40 antibody LAMR 1 antibody LamR antibody LAMR1 antibody LBP antibody LBP/p40 antibody LRP antibody LRP/LR antibody Multidrug resistance associated protein MGr1 Ag antibody Multidrug resistance associated protein MGr1Ag antibody Multidrug resistance-associated protein MGr1-Ag antibody NEM/1CHD4 antibody p40 antibody Ribosomal Protein SA antibody rpsA antibody RSSA_HUMAN antibody SA antibody |
|---|---|
| Molecular Weight(MW) | 40 kDa |
| Cellular Localization | Cell membrane, Nucleus, Cytoplasm. |
Application
Fig1: Western blot analysis of RPSA on different lysates using anti-RPSA antibody at 1/1,000 dilution. Positive control: Lane 1: K562 Lane 2: HepG2 Lane 3: A431
Application
Fig2: ICC staining RPSA in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig3: ICC staining RPSA in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig4: ICC staining RPSA in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig5: ICC staining RPSA in RH-35 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig6: Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-RPSA antibody. Counter stained with hematoxylin.
Application
Fig7: Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using anti-RPSA antibody. Counter stained with hematoxylin.
Application
Fig8: Immunohistochemical analysis of paraffin-embedded human gastric carcinoma tissue using anti-RPSA antibody. Counter stained with hematoxylin.
Application
Fig9: Flow cytometric analysis of MCF-7 cells with RPSA antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.| Positive Control | K562, A431, RH-35, MCF-7, Hela, HepG2, human gastric carcinoma tissue, mouse kidney tissue, mouse brain tissue. |
|---|---|
| Application Notes | WB:1:1,000-1:2,000 ICC:1:100-1:500 IHC:1:50-1:200 FC:1:50-1:100 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
