Application
Fig1: Western blot analysis of Glucocorticoid Receptor on different lysates using anti-Glucocorticoid Receptor antibody at 1/1,000 dilution. Positive control: Lane 1: zebrafish Lane 2: A549 Lane 3: HepG2Application
Fig2: Flow cytometric analysis of NIH/3T3 cells with Glucocorticoid Receptor antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibodyApplication
Fig3:Western blot analysis of Glucocorticoid Receptor on hybrid fish (crucian-carp) heart tissue lysate using anti-Glucocorticoid Receptor antibody at 1/500 dilution.| Product Name | Glucocorticoid Receptor [JF0952] |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | The glucocorticoid receptor (GR) is a ubiquitously expressed transcription factor that mediates the effects of glucocorticoids. The most abundant isoform is GR α. GR induces or represses the expression of genes in response to glucocorticoids, mediating such processes as apoptosis, cell growth and differentiation. A significant class of genes suppressed by GR is controlled by the transcription factor AP-1. GR has also been shown to be the limiting factor in the induction of gene expression by glucocorticoids. It has been revealed that GR forms a complex with HSP 90, rendering the non-ligand bound receptor transcriptionally inactive. More importantly, mutant GRs lacking the signaling domain remain constitutively active. |
| Immunogen | recombinant protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Recombinant rabbit |
| Tested Applications | WB,FC |
| WB:1:1,000-1:5,000 FC:1:50-1:100 |
|
| Species Reactivity | Human,Mouse,Rat,Zebra Fish |
| Concentration | 1mg/ml |
| Purification | Unpurified |
| Alternative Names | GCCR antibody
GCR antibody
GCR_HUMAN antibody
GCRST antibody
glucocorticoid nuclear receptor variant 1 antibody
Glucocorticoid receptor antibody
GR antibody
GRL antibody
Grl1 antibody
nr3c1 antibody
Nuclear receptor subfamily 3 group C member 1 antibody
nuclear receptor subfamily 3 group C member 1 (glucocorticoid receptor) antibody |
|---|---|
| Molecular Weight(MW) | 86 kDa |
| Cellular Localization | Cytoplasm, Nucleus, Mitochondrion. |
Application
Fig1: Western blot analysis of Glucocorticoid Receptor on different lysates using anti-Glucocorticoid Receptor antibody at 1/1,000 dilution. Positive control: Lane 1: zebrafish Lane 2: A549 Lane 3: HepG2
Application
Fig2: Flow cytometric analysis of NIH/3T3 cells with Glucocorticoid Receptor antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody
Application
Fig3:Western blot analysis of Glucocorticoid Receptor on hybrid fish (crucian-carp) heart tissue lysate using anti-Glucocorticoid Receptor antibody at 1/500 dilution.| Positive Control | A549, HepG2, zebrafish. |
|---|---|
| Application Notes | WB:1:1,000-1:5,000 FC:1:50-1:100 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
