Application
Fig1: Western blot analysis of Phospho-PKC alpha(T638) on different lysates using anti-Phospho-PKC alpha(T638) antibody at 1/1,000 dilution. Positive control: Lane 1: Hela Lane 2: 293Application
Fig2: ICC staining Phospho-PKC alpha(T638) in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig3: ICC staining Phospho-PKC alpha(T638) in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig4: ICC staining Phospho-PKC alpha(T638) in SH-SY-5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig5: Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-Phospho-PKC alpha(T638) antibody. Counter stained with hematoxylin.Application
Fig6: Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-Phospho-PKC alpha(T638) antibody. Counter stained with hematoxylin.Application
Fig7: Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-Phospho-PKC alpha(T638) antibody. Counter stained with hematoxylin.| Product Name | Phospho-PKC alpha (T638) [JF0964] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | AAG6 antibody Aging associated gene 6 antibody aPKC antibody KPCA_HUMAN antibody PKC alpha antibody PKC-A antibody PKC-alpha antibody PKCA antibody PRKACA antibody PRKCA antibody Protein Kinase C alpha antibody Protein kinase C alpha type antibody |
| Product description | Members of the protein kinase C (PKC) family play a key regulatory role in a variety of cellular functions including cell growth and differentiation, gene expression, hormone secretion and membrane function. PKCs were originally identified as serine/threonine protein kinases whose activity was dependent on calcium and phospholipids. Diacylglycerols (DAG) and tumor-promoting phorbol esters bind to and activate PKC. PKCs can be subdivided into many different isoforms (α, βI, βII, γ, δ, ε, ζ, η, θ, λ/ι, μ and ν). Patterns of expression for each PKC isoform differ among tissues and PKC family members exhibit clear differences in their cofactor dependencies. For instance, the kinase activities of PKC δ and ε are independent of Ca;2+;. On the other hand, most of the other PKC members possess phorbol ester-binding activities and kinase activities. |
| Immunogen | Synthetic phospho-peptide corresponding to residues surrounding Thr638 of human PKC alpha. |
| Modification | p-T638 |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Recombinant rabbit |
| Tested Applications | WBICC/IFIHCIP |
| WB:1:1,000-1:5,000 ICC:1:50-1:500 IHC:1:50-1:500 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Alternative Names | AAG6 antibody Aging associated gene 6 antibody aPKC antibody KPCA_HUMAN antibody PKC alpha antibody PKC-A antibody PKC-alpha antibody PKCA antibody PRKACA antibody PRKCA antibody Protein Kinase C alpha antibody Protein kinase C alpha type antibody |
|---|---|
| Molecular Weight(MW) | 77 kDa |
| Cellular Localization | Cytoplasm, Cell membrane, Mitochondrion membrane, Nucleus. |
Application
Fig1: Western blot analysis of Phospho-PKC alpha(T638) on different lysates using anti-Phospho-PKC alpha(T638) antibody at 1/1,000 dilution. Positive control: Lane 1: Hela Lane 2: 293
Application
Fig2: ICC staining Phospho-PKC alpha(T638) in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig3: ICC staining Phospho-PKC alpha(T638) in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig4: ICC staining Phospho-PKC alpha(T638) in SH-SY-5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig5: Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-Phospho-PKC alpha(T638) antibody. Counter stained with hematoxylin.
Application
Fig6: Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-Phospho-PKC alpha(T638) antibody. Counter stained with hematoxylin.
Application
Fig7: Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-Phospho-PKC alpha(T638) antibody. Counter stained with hematoxylin.| Positive Control | SH-SY-5Y, MCF-7, Hela, 293, rat brain tissue, human breast carcinoma tissue, mouse brain tissue. |
|---|---|
| Application Notes | WB:1:1,000-1:5,000 ICC:1:50-1:500 IHC:1:50-1:500 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
