Application
Fig1: ICC staining Acetyl CoA synthetase in RH-35 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig2: ICC staining Acetyl CoA synthetase in SW480 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.| Product Name | Acetyl CoA synthetase [JF0917] |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | ACSS2 (acyl-CoA synthetase short-chain family member 2), also known as ACAS2, ACS, ACSA or AceCS, is a 701 amino acid cytoplasmic protein that belongs to the ATP-dependent AMP-binding enzyme family. Existing as a monomer, ACSS2 functions to catalyze the ATP-dependent activation of acetate, a reaction that yields acetyl-CoA for use in energy generation and lipid synthesis. ACSS2 expression, which is highest in liver and kidney tissue, is regulated by the presence of unsaturated fatty acids and sterol regulatory element-binding proteins (SREBPs). Human ACSS2 exists as two alternatively spliced isoforms and shares 93% sequence identity with its mouse counterpart, suggesting a conserved role between species. |
| Immunogen | recombinant protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Recombinant rabbit |
| Tested Applications | WB,ICC/IF |
| WB:1:1,000 ICC:1:50-1:200 |
|
| Species Reactivity | Human,Mouse,Rat |
| Concentration | 1mg/ml |
| Purification | Unpurified |
| Alternative Names | ACAS2 antibody AceCS antibody Acetate CoA ligase antibody Acetate thiokinase antibody AcetateCoA ligase antibody Acetyl CoA synthetase antibody Acetyl Coenzyme A synthetase 2 (ADP forming) antibody Acetyl coenzyme A synthetase cytoplasmic antibody Acetyl-CoA synthetase antibody Acetyl-coenzyme A synthetase antibody ACS antibody ACSA antibody ACSA_HUMAN antibody ACSS2 antibody Acyl activating enzyme antibody Acyl CoA synthetase short chain family member 2 antibody Acyl-activating enzyme antibody Acyl-CoA synthetase short-chain family member 2 antibody Cytoplasmic acetyl coenzyme A synthetase antibody cytoplasmic antibody MYH7B antibody |
|---|---|
| Molecular Weight(MW) | 79 kDa |
| Cellular Localization | Cytoplasm, Nucleus. |
Application
Fig1: ICC staining Acetyl CoA synthetase in RH-35 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig2: ICC staining Acetyl CoA synthetase in SW480 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.| Positive Control | RH-35, SW480. |
|---|---|
| Application Notes | WB:1:1,000 ICC:1:50-1:200 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
