Application
Fig1: Western blot analysis of S100 alpha 6 on different lysates using anti-S100 alpha 6 antibody at 1/1,000 dilution. Positive control: Lane 1: A431 Lane 2: Mouse lungApplication
Fig2: ICC staining S100 alpha 6 in Hela cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig3: ICC staining S100 alpha 6 in A431 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig4: ICC staining S100 alpha 6 in SH-SY-5Y cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig5: Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-S100 alpha 6 antibody. Counter stained with hematoxylin.Application
Fig6: Immunohistochemical analysis of paraffin-embedded human gastric carcinoma tissue using anti-S100 alpha 6 antibody. Counter stained with hematoxylin.Application
Fig7: Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-S100 alpha 6 antibody. Counter stained with hematoxylin.Application
Fig8: Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-S100 alpha 6 antibody. Counter stained with hematoxylin.Application
Fig9: Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using anti-S100 alpha 6 antibody. Counter stained with hematoxylin.Application
Fig10: Flow cytometric analysis of SH-SY-5Y cells with S100 alpha 6 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody| Product Name | S100 alpha 6 [JF0976] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | 2A9 antibody 5B10 antibody CABP antibody CACY antibody Calcyclin antibody Growth factor inducible protein 2A9 antibody Growth factor-inducible protein 2A9 antibody MLN 4 antibody MLN4 antibody OTTHUMP00000015472 antibody OTTHUMP00000015473 antibody PRA antibody PRAGrowth factor inducible protein 2A9 antibody Prolactin receptor associated protein antibody Prolactin receptor-associated protein antibody Protein S100 A6 antibody Protein S100-A6 antibody S100 A6 antibody S100 calcium binding protein A6 (calcyclin) antibody S100 calcium binding protein A6 antibody S100 calcium-binding protein A6 antibody S100A6 antibody S10A6_HUMAN antibody |
| Product description | Calcyclin, also known as Prolactin receptor-associated protein (PRA), growth factor-inducible protein 2A9, S-100 calcium-binding protein A6 (S-100A6) or MLN 4, is a homodimeric member of the S-100 calcium-binding protein family whose expression is upregulated in proliferating and differentiating cells. Calcyclin is inducible by growth factors and overexpressed in acute myeloid leukemias. It is expressed in a cell-specific manner in subpopulations of neurons and astrocytes and in epithelial cells and fibroblasts. Calcyclin is a specific target of S-100B protein in vivo. The binding of Calcyclin to S-100B is stabilized by S-100B-bound calcium and zinc. Calcyclin associates with both Annexin XI and CacyBP (calcyclin-binding protein). It functions to activate several processes along the calcium signal transduction pathway including the regulation of cell growth, proliferation, secretion and exocytosis. |
| Immunogen | recombinant protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Recombinant rabbit |
| Tested Applications | WBICC/IFIHCIPFC |
| WB:1:1,000-1:5,000 ICC:1:100-1:500 IHC:1:50-1:200 FC:1:50-1:100 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Alternative Names | 2A9 antibody 5B10 antibody CABP antibody CACY antibody Calcyclin antibody Growth factor inducible protein 2A9 antibody Growth factor-inducible protein 2A9 antibody MLN 4 antibody MLN4 antibody OTTHUMP00000015472 antibody OTTHUMP00000015473 antibody PRA antibody PRAGrowth factor inducible protein 2A9 antibody Prolactin receptor associated protein antibody Prolactin receptor-associated protein antibody Protein S100 A6 antibody Protein S100-A6 antibody S100 A6 antibody S100 calcium binding protein A6 (calcyclin) antibody S100 calcium binding protein A6 antibody S100 calcium-binding protein A6 antibody S100A6 antibody S10A6_HUMAN antibody |
|---|---|
| Molecular Weight(MW) | 10 kDa |
| Cellular Localization | Nucleus envelope, Cytoplasm, Cell membrane. |
Application
Fig1: Western blot analysis of S100 alpha 6 on different lysates using anti-S100 alpha 6 antibody at 1/1,000 dilution. Positive control: Lane 1: A431 Lane 2: Mouse lung
Application
Fig2: ICC staining S100 alpha 6 in Hela cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig3: ICC staining S100 alpha 6 in A431 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig4: ICC staining S100 alpha 6 in SH-SY-5Y cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig5: Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-S100 alpha 6 antibody. Counter stained with hematoxylin.
Application
Fig6: Immunohistochemical analysis of paraffin-embedded human gastric carcinoma tissue using anti-S100 alpha 6 antibody. Counter stained with hematoxylin.
Application
Fig7: Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-S100 alpha 6 antibody. Counter stained with hematoxylin.
Application
Fig8: Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-S100 alpha 6 antibody. Counter stained with hematoxylin.
Application
Fig9: Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using anti-S100 alpha 6 antibody. Counter stained with hematoxylin.
Application
Fig10: Flow cytometric analysis of SH-SY-5Y cells with S100 alpha 6 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody| Positive Control | A431, SH-SY-5Y, Hela, human colon cancer tissue, human kidney tissue, mouse kidney tissue, human gastric carcinoma tissue, mouse brain tissue. |
|---|---|
| Application Notes | WB:1:1,000-1:5,000 ICC:1:100-1:500 IHC:1:50-1:200 FC:1:50-1:100 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
