Application
Fig1: Western blot analysis of LYVE1 on MCF-7 lysates using anti-LYVE1 antibody at 1/1,000 dilution.Application
Fig2: ICC staining LYVE1 in A431 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig3: ICC staining LYVE1 in HUVEC cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig4: ICC staining LYVE1 in SW480 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig5: Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-LYVE1 antibody. Counter stained with hematoxylin.Application
Fig6: Flow cytometric analysis of HUVEC cells with LYVE1 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody| Product Name | LYVE1 [JF0979] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Cell surface retention sequence-binding protein 1 antibody CRSBP 1 antibody CRSBP-1 antibody CRSBP1 antibody extracellular link domain containing 1 antibody extracellular link domain-containing 1 antibody Extracellular link domain-containing protein 1 antibody HAR antibody Hyaluronic acid receptor antibody Lymphatic endothelium specific hyaluronan receptor antibody lymphatic vessel endothelial hyaluronan receptor 1 antibody Lymphatic vessel endothelial hyaluronic acid receptor 1 antibody LYVE 1 antibody LYVE-1 antibody LYVE1 antibody LYVE1_HUMAN antibody XLKD1 antibody |
| Product description | Lymphatic vessel endothelial hyaluronan receptor-1 (LYVE-1) is expressed on the cell surface as a protein that is reduced by glycosidase treatment. LYVE-1 is abundant in spleen, lymph node, heart, lung and fetal liver, and is less abundant in appendix, bone marrow, placenta, muscle and adult liver. Expression of LYVE-1 is largely restricted to endothelial cells lining lymphatic vessels and splenic sinusoidal endothelial cells. LYVE-1 binds to both soluble and immobilized hyaluronan (HA) with greater specificity than HCAM. Like HCAM, the LYVE-1 molecule binds both soluble and immobilized HA. However, unlike HCAM, the LYVE-1 molecule co-localizes with HA on the luminal face of the lymph vessel wall and is completely absent from blood vessels. Hence, LYVE-1 is the first lymph-specific HA receptor to be characterized and is a uniquely powerful marker for lymph vessels themselves. LYVE-1 is used as a marker to study tumor lymphangiogenesis, which is an important area of investigation. |
| Immunogen | recombinant protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Recombinant rabbit |
| Tested Applications | WBICC/IFIHCFC |
| WB:1:1,000 ICC:1:50-1:200 IHC:1:50-1:200 FC:1:50-1:100 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Alternative Names | Cell surface retention sequence-binding protein 1 antibody CRSBP 1 antibody CRSBP-1 antibody CRSBP1 antibody extracellular link domain containing 1 antibody extracellular link domain-containing 1 antibody Extracellular link domain-containing protein 1 antibody HAR antibody Hyaluronic acid receptor antibody Lymphatic endothelium specific hyaluronan receptor antibody lymphatic vessel endothelial hyaluronan receptor 1 antibody Lymphatic vessel endothelial hyaluronic acid receptor 1 antibody LYVE 1 antibody LYVE-1 antibody LYVE1 antibody LYVE1_HUMAN antibody XLKD1 antibody |
|---|---|
| Molecular Weight(MW) | 35 kDa |
| Cellular Localization | Membrane. |
Application
Fig1: Western blot analysis of LYVE1 on MCF-7 lysates using anti-LYVE1 antibody at 1/1,000 dilution.
Application
Fig2: ICC staining LYVE1 in A431 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig3: ICC staining LYVE1 in HUVEC cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig4: ICC staining LYVE1 in SW480 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig5: Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-LYVE1 antibody. Counter stained with hematoxylin.
Application
Fig6: Flow cytometric analysis of HUVEC cells with LYVE1 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody| Positive Control | A431, MCF-7, HUVEC, SW480, human spleen tissue. |
|---|---|
| Application Notes | WB:1:1,000 ICC:1:50-1:200 IHC:1:50-1:200 FC:1:50-1:100 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
