WB
Western blot analysis of Thymidine Kinase 1 / TK1 on different lysates with Rabbit anti-Thymidine Kinase 1 / TK1 antibody at 1/1,000 dilution. Lane 1: HeLa cell lysate, Lane 2: A549 cell lysate, Lysates/proteins at 20 µg/Lane. Exposure time: 1 minute; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.IHC
Immunohistochemical analysis of paraffin-embedded human tonsil tissue with Rabbit anti-Thymidine Kinase 1 / TK1 antibody at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.| Product Name | Thymidine Kinase 1 / TK1 Recombinant Rabbit Monoclonal Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Thymidine Kinase (TK1) is a highly conserved phosphotransferase that is present in most living cells. Thymidine Kinase catalyzes the phosphorylation reaction: deoxythymidine + ATP = deoxythymidine 5'-phosphate + ADP; it is thus involved in the reaction chain to introduce deoxythymidine into the DNA. Thymidine kinase is required for the action of many antiviral drugs, such as azidothymidine (AZT), and is is also used to select hybridoma cell lines in the production of monoclonal antibodies. Thymidine Kinase has many clinical applications as it is only present in anticipation of cell division. Because of this, Thymidine Kinase can be used as a proliferation marker in the diagnosis, treatment, and follow-up of malignant diseases, especially hematological malignancies. Thymidine Kinase may be observed as a monomer, dimer, trimer or tetramer. |
| Immunogen | Synthetic peptide within Human Thymidine Kinase 1 aa 2-49 / 234. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Recombinant rabbit |
| Tested Applications | IHCWB |
| WB:1:1000 IHC:1:1000 |
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| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Alternative Names | cytosolic antibody KITH_HUMAN antibody Thymidine kinase 1 antibody Thymidine kinase 1 soluble antibody Thymidine kinase 1 soluble isoform antibody Thymidine kinase antibody Thymidine kinase cytosolic antibody TK 1 antibody TK 2 antibody TK1 antibody Tk1a antibody Tk1b antibody TK2 antibody |
|---|---|
| Molecular Weight(MW) | 25 kDa |
| Cellular Localization | Cytoplasm. |
| SwissProt ID | P04183 |
|---|
WB
Western blot analysis of Thymidine Kinase 1 / TK1 on different lysates with Rabbit anti-Thymidine Kinase 1 / TK1 antibody at 1/1,000 dilution. Lane 1: HeLa cell lysate, Lane 2: A549 cell lysate, Lysates/proteins at 20 µg/Lane. Exposure time: 1 minute; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.
IHC
Immunohistochemical analysis of paraffin-embedded human tonsil tissue with Rabbit anti-Thymidine Kinase 1 / TK1 antibody at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.| Application Notes | WB:1:1000 IHC:1:1000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
