Application
Fig1: Western blot analysis of MHC class I on different lysates using anti-MHC class I antibody at 1/1,000 dilution. Positive control: Lane 1: Raji Lane 2: THP-1Application
Fig2: ICC staining MHC class I in SH-SY-5Y cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig3: Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-MHC class I antibody. Counter stained with hematoxylin.Application
Fig4: Flow cytometric analysis of THP-1 cells with MHC class I antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody| Product Name | MHC class I [JF10-38] |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Major histocompatibility complex (MHC) molecules, also designated human leukocyte antigen (HLA) molecules, are cell-surface receptors that bind foreign peptides and present them to T lymphocytes. MHC class I molecules consist of two polypeptide chains, an a or heavy chain, and β-2-Microglobulin, a non-covalently associated protein. Cytotoxic T lymphocytes bind antigenic peptides presented by MHC class I molecules. Antigens that bind to MHC class I molecules are typically 8-10 residues in length and are stabilized in a peptide binding groove. MHC class II molecules are encoded by polymorphic MHC genes and consist of a non-covalent complex of an a and b chain. Helper T lymphocytes bind antigenic peptides presented by MHC class II molecules. MHC class II molecules bind 13-18 amino acid antigenic peptides. Accumulating in endosomal/lysosomal compartments and on the surface of B cells, HLA-DM and -DO molecules regulate binding of exogenous peptides to class II molecules (HLA-DR) by sustaining a conformation that favors peptide exchange. The differential structural properties of MHC class I and class II molecules account for their respective roles in activating different populations of T lymphocytes. |
| Immunogen | recombinant protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Recombinant rabbit |
| Tested Applications | WB,ICC/IF,IHC,FC |
| WB:1:1,000-1:5,000 ICC:1:100-1:500 IHC:1:50-1:200 FC:1:50-1:100 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Unpurified |
| Alternative Names | A 28 antibody
A 9 antibody
Antigen presenting molecule antibody Aw 24 antibody Aw 68 antibody CLASS I HISTOCOMPATIBILITY ANTIGEN antibody H2 K1 antibody H2K antibody HLA A antibody HLA class I histocompatibility antigen A 1 alpha chain antibody HLA class I histocompatibility antigen A 11 alpha chain antibody HLA class I histocompatibility antigen A 2 alpha chain antibody HLA class I histocompatibility antigen A 24 alpha chain antibody HLA class I histocompatibility antigen A 3 alpha chain antibody HLA class I histocompatibility antigen A 30 alpha chain antibody HLA class I histocompatibility antigen A 32 alpha chain antibody HLA class I histocompatibility antigen A 68 alpha chain antibody HLAA antibody Leukocyte antigen class I A antibody Major histocompatibility complex class I A antibody MHC class I antigen A 1 antibody MHC class I antigen A*11 antibody MHC class I antigen A*2 antibody MHC class I antigen A*24 antibody MHC class I antigen A*3 antibody MHC class I antigen A*30 antibody MHC class I antigen A*32 antibody MHC class I antigen A*68 antibody MHC class I antigen HLA A heavy chain antibody MHC class I heavy chain H2 K antibody |
|---|---|
| Molecular Weight(MW) | 41 kDa |
| Cellular Localization | Cell Membrane,Type I membrane protein. |
Application
Fig1: Western blot analysis of MHC class I on different lysates using anti-MHC class I antibody at 1/1,000 dilution. Positive control: Lane 1: Raji Lane 2: THP-1
Application
Fig2: ICC staining MHC class I in SH-SY-5Y cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig3: Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-MHC class I antibody. Counter stained with hematoxylin.
Application
Fig4: Flow cytometric analysis of THP-1 cells with MHC class I antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody| Positive Control | Raji, THP-1, SH-SY-5Y, human spleen tissue. |
|---|---|
| Application Notes | WB:1:1,000-1:5,000 ICC:1:100-1:500 IHC:1:50-1:200 FC:1:50-1:100 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
