Application
Fig1: Western blot analysis of Galectin 3 on different lysates using anti-Galectin 3 antibody at 1/1,000 dilution. Positive control: Lane 1: Hela Lane 2: NIH/3T3 Lane 3: THP-1Application
Fig2: ICC staining Galectin 3 in Hela cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig3: ICC staining Galectin 3 in NIH/3T3 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig4: Flow cytometric analysis of Hela cells with Galectin 3 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody| Product Name | Galectin 3 [JF39-10] |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Galectins are a family of soluble b-galactoside-binding animal lectins that modulate cell-to-cell adhesion and cell-to-extracellular matrix (ECM) inter- actions and play a role in tumor progression, pre-mRNA splicing and apop-tosis. The galectin-3 protein, also known as Mac-2, hMac-2, GALBP, CBP35 or LGALS3, contains a single carbohydrate binding domain, which binds galactose-containing glycoconjugates. Galectin-3 is expressed in colonic and intestinal epithelium, inflammatory macrophages, papillary and follicular carcinomas, neoplastic astrocytes and some B and T lymphocytes. Upregulated expression of galectin-3 is involved in cancer progression and metastasis. Galectin-3 mediates the endocytosis of β1 Integrins in a lactose-dependent manner and is associated with thyroid malignancy and Crohn's disease. It may also be used as a marker for diagnosing cases involving Hurthle cell adenomas and carcinomas. |
| Immunogen | recombinant protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Recombinant rabbit |
| Tested Applications | WB,ICC/IF,FC |
| WB:1:1,000-1:5,000 FC:1:50-1:100 ICC:1:50-1:200 |
|
| Species Reactivity | Human,Rat |
| Concentration | 1mg/ml |
| Purification | Unpurified |
| Alternative Names | 35 kDa lectin antibody
Carbohydrate binding protein 35 antibody
Carbohydrate-binding protein 35 antibody
CBP 35 antibody
CBP35 antibody
Gal-3 antibody
GAL3 antibody
Galactose-specific lectin 3 antibody
Galactoside-binding protein antibody
GALBP antibody
Galectin 3 internal gene included antibody Galectin-3 antibody Galectin3 antibody GALIG antibody GBP antibody IgE binding protein antibody IgE-binding protein antibody L 31 antibody L 34 antibody L-31 antibody L-34 galactoside-binding lectin antibody L31 antibody Laminin-binding protein antibody Lectin L-29 antibody Lectin galactose binding soluble 3 antibody LEG3_HUMAN antibody LGALS2 antibody LGALS3 antibody MAC 2 antigen antibody Mac-2 antibody Mac-2 antigen antibody MAC2 antibody Macrophage galactose-specific lectin antibody MGC105387 antibody |
|---|---|
| Molecular Weight(MW) | 26 kDa |
| Cellular Localization | Cytoplasm, Nucleus, Secreted. |
Application
Fig1: Western blot analysis of Galectin 3 on different lysates using anti-Galectin 3 antibody at 1/1,000 dilution. Positive control: Lane 1: Hela Lane 2: NIH/3T3 Lane 3: THP-1
Application
Fig2: ICC staining Galectin 3 in Hela cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig3: ICC staining Galectin 3 in NIH/3T3 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig4: Flow cytometric analysis of Hela cells with Galectin 3 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody| Positive Control | NIH/3T3, THP-1, Hela. |
|---|---|
| Application Notes | WB:1:1,000-1:5,000 FC:1:50-1:100 ICC:1:50-1:200 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
