Application
Fig1: Western blot analysis of NQO1 on different lysates using anti-NQO1 antibody at 1/1,000 dilution. Positive control: Lane 1: SH-SY-5Y Lane 2: Mouse kidney Lane 3: RajiApplication
Fig2: Flow cytometric analysis of Hela cells with NQO1 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody| Product Name | NQO1 [JF440-1] |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | NAD(P)H:quinone oxidoreductase 1 (NQO1) and NRH:quinone oxidoreductase (NQO2) are flavoproteins that catalyze the metabolic detoxification of quinones and their derivatives to hydroquinones, using either NADH or NADPH as the electron donor. This protects cells against quinone-induced oxidative stress, cytotoxicity, and mutagenicity. Many tumors overexpress NQO1, which is an obligate two-electron reductase that deactivates toxins and activates bioreductive anticancer drugs. NQO1, a 274 amino acid protein, is ubiquitously expressed, but the expression level varies among tissues. NQO1 gene expression is coordinately induced in response to xenobiotics, antioxidants, heavy metals and radiation. The antioxidant response element (ARE) in the NQO1 gene promoter is essential for expression and coordinated induction of NQO1. ARE activation by tert-butylhydroquinone is dependent on PI3-kinase, which lies upstream of Nrf2. Nrf2, c-Jun, Nrf1, Jun-B and Jun-D bind to the ARE and regulate expression and induction of NQO1 gene. Maf-Maf homodimers and possibly Maf-Nrf2 heterodimers play a role in negative regulation of ARE-mediated transcription, but Maf-Nrf1 heterodimers fail to bind with the NQO1 gene ARE and do not repress NQO1 transcription. |
| Immunogen | recombinant protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Recombinant rabbit |
| Tested Applications | WB,ICC/IF,IP,FC |
| WB:1:1,000-1:5,000 FC:1:50-1:100 ICC:1:50-1:200 IP:1:50 |
|
| Species Reactivity | Human,Mouse,Rat |
| Concentration | 1mg/ml |
| Purification | Unpurified |
| Alternative Names | Azoreductase antibody Cytochrome b 5 reductase antibody DHQU antibody DIA 4 antibody DIA4 antibody Diaphorase (NADH/NADPH) (cytochrome b 5 reductase) antibody Diaphorase (NADH/NADPH) antibody Diaphorase 4 antibody Dioxin inducible 1 antibody DT diaphorase antibody DT-diaphorase antibody DTD antibody Menadione reductase antibody NAD(P)H dehydrogenase [quinone] 1 antibody NAD(P)H dehydrogenase quinone 1 antibody NAD(P)H menadione oxidoreductase 1 dioxin inducible antibody NAD(P)H: menadione oxidoreductase 1 dioxin inducible 1 antibody NAD(P)H:menadione oxidoreductase 1 antibody NAD(P)H:Quinone acceptor oxidoreductase type 1 antibody NAD(P)H:quinone oxidoreductase 1 antibody NAD(P)H:quinone oxireductase antibody NMOR 1 antibody NMOR I antibody NMOR1 antibody NMORI antibody NQO 1 antibody NQO1 antibody NQO1_HUMAN antibody Phylloquinone reductase antibody QR 1 antibody QR1 antibody Quinone reductase 1 antibody |
|---|---|
| Molecular Weight(MW) | 31/27 kDa |
| Cellular Localization | Cytoplasm. |
Application
Fig1: Western blot analysis of NQO1 on different lysates using anti-NQO1 antibody at 1/1,000 dilution. Positive control: Lane 1: SH-SY-5Y Lane 2: Mouse kidney Lane 3: Raji
Application
Fig2: Flow cytometric analysis of Hela cells with NQO1 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody| Positive Control | Raji, SH-SY-5Y, mouse kidney tissue. |
|---|---|
| Application Notes | WB:1:1,000-1:5,000 FC:1:50-1:100 ICC:1:50-1:200 IP:1:50 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
