Application
Fig1: Western blot analysis of CD147 on different lysates using anti-CD147 antibody at 1/1,000 dilution. Positive control: Lane 1: Hela Lane 2: Jurkat Lane 3: Mouse kidneyApplication
Fig2: ICC staining CD147 in Hela cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig3: ICC staining CD147 in A431 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig4: ICC staining CD147 in SKOV-3 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig5: Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-CD147 antibody. Counter stained with hematoxylin.Application
Fig6: Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-CD147 antibody. Counter stained with hematoxylin.Application
Fig7: Immunohistochemical analysis of paraffin-embedded mouse heart tissue using anti-CD147 antibody. Counter stained with hematoxylin.Application
Fig8: Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-CD147 antibody. Counter stained with hematoxylin.Application
Fig9: Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-CD147 antibody. Counter stained with hematoxylin.| Product Name | CD147 [JF1-045] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | 5A11 antigen antibody 5F7 antibody BASI_HUMAN antibody Basigin (Ok blood group) antibody Basigin antibody Blood brain barrier HT7 antigen antibody Bsg antibody CD 147 antibody CD147 antibody CD147 antigen antibody Collagenase stimulatory factor antibody EMMPRIN antibody Extracellular matrix metalloproteinase inducer antibody Leukocyte activation antigen M6 antibody M 6 antibody M6 antibody M6 leukocyte activation antigen antibody Neurothelin antibody OK antibody OK blood group antibody OK blood group antigen antibody TCSF antibody Tumor cell derived collagenase stimulatory factor antibody Tumor cell-derived collagenase stimulatory factor antibody |
| Product description | Extracellular matrix metalloproteinase inducer (EMMPRIN), also designated basigin or CD147, is involved in the regulation of matrix remodeling at the epidermal-dermal interface. EMMPRIN stimulates the production of interstitial collagenase, gelatinase A, stromelysin-1 and various metalloproteinases (MMPs) by fibroblasts. These enzymes, which are typically increased during tissue degradation and wound healing, are important factors in cancer invasion and metastasis. |
| Immunogen | recombinant protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Recombinant rabbit |
| Tested Applications | WBICC/IFIHC |
| WB:1:1,000-1:2,000 ICC:1:50-1:200 IHC:1:50-1:200 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Alternative Names | 5A11 antigen antibody 5F7 antibody BASI_HUMAN antibody Basigin (Ok blood group) antibody Basigin antibody Blood brain barrier HT7 antigen antibody Bsg antibody CD 147 antibody CD147 antibody CD147 antigen antibody Collagenase stimulatory factor antibody EMMPRIN antibody Extracellular matrix metalloproteinase inducer antibody Leukocyte activation antigen M6 antibody M 6 antibody M6 antibody M6 leukocyte activation antigen antibody Neurothelin antibody OK antibody OK blood group antibody OK blood group antigen antibody TCSF antibody Tumor cell derived collagenase stimulatory factor antibody Tumor cell-derived collagenase stimulatory factor antibody |
|---|---|
| Molecular Weight(MW) | 42 kDa |
| Cellular Localization | Cell membrane, Melanosome. |
Application
Fig1: Western blot analysis of CD147 on different lysates using anti-CD147 antibody at 1/1,000 dilution. Positive control: Lane 1: Hela Lane 2: Jurkat Lane 3: Mouse kidney
Application
Fig2: ICC staining CD147 in Hela cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig3: ICC staining CD147 in A431 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig4: ICC staining CD147 in SKOV-3 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig5: Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-CD147 antibody. Counter stained with hematoxylin.
Application
Fig6: Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-CD147 antibody. Counter stained with hematoxylin.
Application
Fig7: Immunohistochemical analysis of paraffin-embedded mouse heart tissue using anti-CD147 antibody. Counter stained with hematoxylin.
Application
Fig8: Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-CD147 antibody. Counter stained with hematoxylin.
Application
Fig9: Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-CD147 antibody. Counter stained with hematoxylin.| Positive Control | A431, SKOV-3, Hela, Jurkat, rat brain tissue, human colon cancer tissue, mouse brain tissue, mouse testis tissue, mouse heart tissue. |
|---|---|
| Application Notes | WB:1:1,000-1:2,000 ICC:1:50-1:200 IHC:1:50-1:200 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
