Application
Fig1: Western blot analysis of NEFH on mouse brain lysates using anti-NEFH antibody at 1/1,000 dilution.Application
Fig2: Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue using anti-NEFH antibody. Counter stained with hematoxylin.Application
Fig3: Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-NEFH antibody. Counter stained with hematoxylin.| Product Name | Neurofilament heavy polypeptide [JF99-07] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | 200 kDa neurofilament protein antibody CMT2CC antibody Nefh antibody Neurofilament heavy polypeptide 200kDa antibody Neurofilament heavy polypeptide antibody Neurofilament triplet H protein antibody NF H antibody NF-H antibody NFH antibody NFH_HUMAN antibody |
| Product description | Neurofilament-H (NF-H), for neurofilament heavy polypeptide, a member of the intermediate filament family, is a major component of neuronal cytoskeletons. Neurofilaments are dynamic structures; they contain phosphorylation sites for a large number of protein kinases, including protein kinase A, protein kinase C, cyclin-dependent kinase 5, extracellular signal regulated kinase, glycogen synthase kinase-3, and stress-activated protein kinase gamma. In addition to their role in the control of axon caliber, neurofilaments may affect other cytoskeletal elements, such as microtubules and Actin filaments. Changes in neurofilament phosphorylation or metabolism are frequently observed in neurodegenerative diseases, including amyotrophic lateral sclerosis (ALS), Parkinson's disease and Alzheimer's disease. |
| Immunogen | recombinant protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Recombinant rabbit |
| Tested Applications | WBIHC |
| WB:1:500-1:1,000 IHC:1:50-1:200 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Alternative Names | 200 kDa neurofilament protein antibody CMT2CC antibody Nefh antibody Neurofilament heavy polypeptide 200kDa antibody Neurofilament heavy polypeptide antibody Neurofilament triplet H protein antibody NF H antibody NF-H antibody NFH antibody NFH_HUMAN antibody |
|---|---|
| Molecular Weight(MW) | 200 kDa |
| Cellular Localization | Cytoplasm. |
Application
Fig1: Western blot analysis of NEFH on mouse brain lysates using anti-NEFH antibody at 1/1,000 dilution.
Application
Fig2: Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue using anti-NEFH antibody. Counter stained with hematoxylin.
Application
Fig3: Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-NEFH antibody. Counter stained with hematoxylin.| Positive Control | Rat cerebellum tissue, mouse brain tissue. |
|---|---|
| Application Notes | WB:1:500-1:1,000 IHC:1:50-1:200 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
