Application
Fig1: ICC staining S100A9 in A549 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig2: ICC staining S100A9 in Hela cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig3: ICC staining S100A9 in HepG2 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig4: Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-S100A9 antibody. Counter stained with hematoxylin.Application
Fig5: Immunohistochemical analysis of paraffin-embedded human liver cancer tissue using anti-S100A9 antibody. Counter stained with hematoxylin.Application
Fig6: Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-S100A9 antibody. Counter stained with hematoxylin.Application
Fig7: Immunohistochemical analysis of paraffin-embedded human lung cancer tissue using anti-S100A9 antibody. Counter stained with hematoxylin.Application
Fig8: Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-S100A9 antibody. Counter stained with hematoxylin.| Product Name | S100A9 [JF096-8] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Leukocyte L1 complex heavy chain antibody 60B8AG antibody CAGB antibody Calgranulin B antibody Calgranulin-B antibody Calprotectin L1H subunit antibody CFAG antibody CGLB antibody Cystic fibrosis antigen B antibody L1AG antibody Leukocyte L1 complex heavy chain antibody LIAG antibody MAC387 antibody MIF antibody Migration inhibitory factor related protein 14 antibody Migration inhibitory factor-related protein 14 antibody MRP 14 antibody MRP-14 antibody MRP14 antibody Myeloid-related protein 14 antibody NIF antibody OTTHUMP00000015331 antibody p14 antibody Protein S100-A9 antibody S100 A9 antibody S100 calcium binding protein A9 antibody S100 calcium binding protein A9 calgranulin B antibody S100 calcium-binding protein A9 antibody S100A9 antibody S10A9_HUMAN antibody |
| Product description | The family of EF-hand type Ca;2+;-binding proteins includes Calbindin (previously designated vitamin D-dependent Ca;2+;-binding protein), S-100α and β, Calgranulin A (also designated MRP8), Calgranulin B (also designated MRP14) and Calgranulin C (S-100 like protein), and the parvalbumin family members, including parvalbumin α and parvalbumin β (also designated oncomodulin). Calbindin, S-100 proteins and parvalbumin proteins are each expressed in neural tissues. In addition, S-100α and β are present in a variety of other tissues, and Calbindin is present in intestine and kidney. Parvalbumin α is also found in fast-contracting/relaxing skeletal muscle fibers and parvalbumin β is found in many tumor tissues as well as in the organ of Corti. Calbindin, S-100 proteins and parvalbulmins have all been detected in leydig cells and testis. These proteins are thought to play a role in hormone production and spermatogenesis. Calgranulin is expressed in macrophages and epithelial cells. |
| Immunogen | recombinant protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Recombinant rabbit |
| Tested Applications | WBICC/IFIHC |
| WB:1:1,000 ICC:1:50-1:200 IHC:1:50-1:200 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Alternative Names | Leukocyte L1 complex heavy chain antibody 60B8AG antibody CAGB antibody Calgranulin B antibody Calgranulin-B antibody Calprotectin L1H subunit antibody CFAG antibody CGLB antibody Cystic fibrosis antigen B antibody L1AG antibody Leukocyte L1 complex heavy chain antibody LIAG antibody MAC387 antibody MIF antibody Migration inhibitory factor related protein 14 antibody Migration inhibitory factor-related protein 14 antibody MRP 14 antibody MRP-14 antibody MRP14 antibody Myeloid-related protein 14 antibody NIF antibody OTTHUMP00000015331 antibody p14 antibody Protein S100-A9 antibody S100 A9 antibody S100 calcium binding protein A9 antibody S100 calcium binding protein A9 calgranulin B antibody S100 calcium-binding protein A9 antibody S100A9 antibody S10A9_HUMAN antibody |
|---|---|
| Molecular Weight(MW) | 14 kDa |
| Cellular Localization | Secreted, Cytoplasm, Cell membrane. |
| SwissProt ID | P06702 |
|---|
Application
Fig1: ICC staining S100A9 in A549 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig2: ICC staining S100A9 in Hela cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig3: ICC staining S100A9 in HepG2 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig4: Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-S100A9 antibody. Counter stained with hematoxylin.
Application
Fig5: Immunohistochemical analysis of paraffin-embedded human liver cancer tissue using anti-S100A9 antibody. Counter stained with hematoxylin.
Application
Fig6: Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-S100A9 antibody. Counter stained with hematoxylin.
Application
Fig7: Immunohistochemical analysis of paraffin-embedded human lung cancer tissue using anti-S100A9 antibody. Counter stained with hematoxylin.
Application
Fig8: Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-S100A9 antibody. Counter stained with hematoxylin.| Positive Control | A549, Hela, HepG2, human tonsil tissue, human lung cancer tissue, human liver cancer tissue, human spleen tissue, human breast carcinoma tissue |
|---|---|
| Application Notes | WB:1:1,000 ICC:1:50-1:200 IHC:1:50-1:200 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
