Application
Fig1: Western blot analysis of Dnmt1 on HepG2 cells lysates using anti-Dnmt1 antibody at 1/1,000 dilution.Application
Fig2: ICC staining Dnmt1 in Hela cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig3: ICC staining Dnmt1 in HepG2 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig4: ICC staining Dnmt1 in 293T cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig5: Immunohistochemical analysis of paraffin-embedded mouse placenta tissue using anti-Dnmt1 antibody. Counter stained with hematoxylin.| Product Name | Dnmt1 [JF09-89] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | ADCADN antibody AIM antibody CXXC finger protein 9 antibody CXXC-type zinc finger protein 9 antibody CXXC9 antibody DNA (cytosine 5 ) methyltransferase 1 antibody DNA (cytosine-5)-methyltransferase 1 antibody DNA methyltransferase 1 antibody DNA methyltransferase HsaI antibody DNA methyltransferase M.HsaI. antibody DNA MTase antibody DNA MTase HsaI antibody DNMT 1 antibody DNMT antibody Dnmt1 antibody DNMT1_HUMAN antibody Dnmt1o antibody FLJ16293 antibody HSN1E antibody M.HsaI antibody MCMT antibody Met1 antibody MGC104992 antibody mMmul antibody MommeD2 antibody |
| Product description | Methylation at the 5'-position of cytosine is the only known naturally occurring covalent modification of the mammalian genome. DNA methylation requires the enzymatic activity of DNA 5-cytosine methyltransferase (Dnmt) proteins, which catalyze the transfer of a methyl group from S-adenosyl methionine to the 5'-position of cytosines residing in the dinucleotide CpG motif, and this methylation results in transcriptional repression of the target gene. The Dnmt enzymes are encoded by independent genes. Dnmt1 is the most abundant, and it preferentially methylates hemimethylated DNA and coordinates gene expression during development. Additional mammalian Dnmt proteins include Dnmt2 and Dnmt3. Dnmt2 lacks the large N-terminal regulator domain of Dnmt1, is expressed at substantially lower levels in adult tissues, and is likely involved in methylating newly integrated retroviral DNA. Dnmt3a and Dnmt3b are encoded by two distinct genes, but both are abundantly expressed in embryonic stem cells, where they also methylate CpG motifs on DNA. |
| Immunogen | recombinant protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Recombinant rabbit |
| Tested Applications | WBICC/IFIHC |
| WB:1:1,000-1:2,000 ICC:1:50-1:200 IHC:1:50-1:200 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Alternative Names | ADCADN antibody AIM antibody CXXC finger protein 9 antibody CXXC-type zinc finger protein 9 antibody CXXC9 antibody DNA (cytosine 5 ) methyltransferase 1 antibody DNA (cytosine-5)-methyltransferase 1 antibody DNA methyltransferase 1 antibody DNA methyltransferase HsaI antibody DNA methyltransferase M.HsaI. antibody DNA MTase antibody DNA MTase HsaI antibody DNMT 1 antibody DNMT antibody Dnmt1 antibody DNMT1_HUMAN antibody Dnmt1o antibody FLJ16293 antibody HSN1E antibody M.HsaI antibody MCMT antibody Met1 antibody MGC104992 antibody mMmul antibody MommeD2 antibody |
|---|---|
| Molecular Weight(MW) | 183 kDa |
| Cellular Localization | Nucleus. |
Application
Fig1: Western blot analysis of Dnmt1 on HepG2 cells lysates using anti-Dnmt1 antibody at 1/1,000 dilution.
Application
Fig2: ICC staining Dnmt1 in Hela cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig3: ICC staining Dnmt1 in HepG2 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig4: ICC staining Dnmt1 in 293T cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig5: Immunohistochemical analysis of paraffin-embedded mouse placenta tissue using anti-Dnmt1 antibody. Counter stained with hematoxylin.| Positive Control | 293T, Hela, HepG2, mouse placenta tissue. |
|---|---|
| Application Notes | WB:1:1,000-1:2,000 ICC:1:50-1:200 IHC:1:50-1:200 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
