Application
Fig1: Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-58K Golgi protein antibody. Counter stained with hematoxylin.Application
Fig2: Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-58K Golgi protein antibody. Counter stained with hematoxylin.Application
Fig3: Flow cytometric analysis of HepG2 cells with 58K Golgi protein antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody| Product Name | 58K Golgi protein [JF099-5] |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | 58K protein antibodies are excellent for use as markers for the Golgi complex. The 58K protein has been identified as being FTCD, a bifunctional enzyme that channels 1-carbon units from formiminoglutamate, a metabolite of the histidine degradation pathway, to the folate pool. Defects in FTCD are the cause of glutamate formiminotransferase deficiency [also known as formiminoglutamicaciduria (FIGLU-uria)], an autosomal recessive disorder. Features of a severe phenotype include elevated levels of formiminoglutamate (FIGLU) in the urine in response to histidine administration, megaloblastic anemia and mental retardation. Features of a mild phenotype include high urinary excretion of FIGLU in the absence of histidine administration, mild developmental delay and no hematological abnormalities. |
| Immunogen | recombinant protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Recombinant rabbit |
| Tested Applications | WB,IHC,FC |
| WB:1:500-1:1,000 FC:1:50-1:100 IHC:1:50-1:200 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Unpurified |
| Alternative Names | Formimidoyltetrahydrofolate cyclodeaminase antibody Formimidoyltransferase cyclodeaminase antibody Formiminotetrahydrofolate cyclodeaminase antibody Formiminotransferase cyclodeaminase antibody Formiminotransferase-cyclodeaminase antibody FTCD antibody FTCD_HUMAN antibody Glutamate formiminotransferase antibody Glutamate formyltransferase antibody LCHC 1 antibody LCHC1 antibody |
|---|---|
| Molecular Weight(MW) | 59 kDa |
| Cellular Localization | Cytoplasm, Golgi apparatus. |
| SwissProt ID | O95954 |
|---|
Application
Fig1: Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-58K Golgi protein antibody. Counter stained with hematoxylin.
Application
Fig2: Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-58K Golgi protein antibody. Counter stained with hematoxylin.
Application
Fig3: Flow cytometric analysis of HepG2 cells with 58K Golgi protein antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody| Positive Control | Human liver tissue, human kidney tissue. |
|---|---|
| Application Notes | WB:1:500-1:1,000 FC:1:50-1:100 IHC:1:50-1:200 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
