Application
Fig1: Western blot analysis of Gli1 on SH-SY-5Y cells lysates using anti-Gli1 antibody at 1/1,000 dilution.Application
Fig2: ICC staining Gli1 in HepG2 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig3: ICC staining Gli1 in SH-SY-5Y cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig4: ICC staining Gli1 in 293T cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig5: Immunohistochemical analysis of paraffin-embedded rat testis tissue using anti-Gli1 antibody. Counter stained with hematoxylin.Application
Fig6: Immunohistochemical analysis of paraffin-embedded mouse fallopian tube tissue using anti-Gli1 antibody. Counter stained with hematoxylin.| Product Name | Gli1 [JF09-08] |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Zinc-finger proteins contain DNA-binding domains and have a wide variety of functions, most of which encompass some form of transcriptional activation or repression. The majority of zinc-finger proteins contain a Kr |
| Immunogen | recombinant protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Recombinant rabbit |
| Tested Applications | WB,ICC,IHC |
| WB:1:500-1:1,000 IHC:1:50-1:200ICC:1:50-1:200 |
|
| Species Reactivity | Human,Mouse,Rat |
| Concentration | 1mg/ml |
| Purification | Unpurified |
| Alternative Names | Gli 1 antibody GLI antibody GLI family zinc finger 1 antibody GLI Kruppel family member 1 antibody gli1 antibody GLI1_HUMAN antibody Glioma associated oncogene 1 antibody Glioma associated oncogene homolog 1 (zinc finger protein) antibody Glioma associated oncogene homolog antibody Glioma-associated oncogene antibody Oncogene GLI antibody Zfp 5 antibody Zfp5 antibody Zinc finger protein GLI 1 antibody Zinc finger protein GLI1 antibody |
|---|---|
| Molecular Weight(MW) | 150 kDa |
| Cellular Localization | Cytoplasm, Nucleus. |
| SwissProt ID | P08151 |
|---|
Application
Fig1: Western blot analysis of Gli1 on SH-SY-5Y cells lysates using anti-Gli1 antibody at 1/1,000 dilution.
Application
Fig2: ICC staining Gli1 in HepG2 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig3: ICC staining Gli1 in SH-SY-5Y cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig4: ICC staining Gli1 in 293T cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig5: Immunohistochemical analysis of paraffin-embedded rat testis tissue using anti-Gli1 antibody. Counter stained with hematoxylin.
Application
Fig6: Immunohistochemical analysis of paraffin-embedded mouse fallopian tube tissue using anti-Gli1 antibody. Counter stained with hematoxylin.| Positive Control | HepG2, SH-SY-5Y, 293T, rat testis tissue, mouse fallopian tube tissue. |
|---|---|
| Application Notes | WB:1:500-1:1,000 IHC:1:50-1:200ICC:1:50-1:200 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
