Application
Fig1: Western blot analysis of MiTF on PC-12 cells lysates using anti-MiTF antibody at 1/1,000 dilution.Application
Fig2: ICC staining MiTF in Hela cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig3: ICC staining MiTF in A431 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig4: ICC staining MiTF in NIH/3T3 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig5: Flow cytometric analysis of SW480 cells with MiTF antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.| Product Name | MiTF [JF100-01] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | BHLHE32 antibody bHLHe32 antibody Class E basic helix-loop-helix protein 32 antibody CMM8 antibody Homolog of mouse microphthalmia antibody Mi antibody Microphthalmia associated transcription factor antibody Microphthalmia, mouse, homolog of antibody Microphthalmia-associated transcription factor antibody MITF antibody MITF_HUMAN antibody mitfa antibody nacre antibody WS2 antibody WS2A antibody z3A.1 antibody |
| Product description | MITF (microphthalmia-associated transcription factor) is a melanocytic nuclear protein that contains basic helix-loop-helix (HLH) and leucine zipper (LZ) domains. These protein motifs are frequently observed in other transcription factors and are particularly common to members of the Myc family. MITF can directly associate with DNA as a homodimer and is required for the development and differentiation of melanocytes. Its expression is upregulated by cAMP and cAMP-dependent pathways. MITF activates several different gene promoters by binding to their E-boxes. Tyrosinase, TRP1 and TRP2 are pigment synthesis genes activated by MITF. When MITF is phosphorylated on Ser73 (via the MAPK pathway), it associates with co-activators of the p300/CBP family and enhances transcription. MITF has several isoforms including MITF-M which is specifically expressed in melanocytes. In MITF-deficient mice there is a complete absence of melanocytes. |
| Immunogen | recombinant protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Recombinant rabbit |
| Tested Applications | WBICCFC |
| WB:1:1,000-1:2,000 FC:1:50-1:100 ICC:1:100-1:500 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Alternative Names | BHLHE32 antibody
bHLHe32 antibody
Class E basic helix-loop-helix protein 32 antibody
CMM8 antibody
Homolog of mouse microphthalmia antibody
Mi antibody
Microphthalmia associated transcription factor antibody
Microphthalmia mouse homolog of antibody Microphthalmia-associated transcription factor antibody MITF antibody MITF_HUMAN antibody mitfa antibody nacre antibody WS2 antibody WS2A antibody z3A.1 antibody |
|---|---|
| Molecular Weight(MW) | 55 kDa |
| Cellular Localization | Nucleus. |
Application
Fig1: Western blot analysis of MiTF on PC-12 cells lysates using anti-MiTF antibody at 1/1,000 dilution.
Application
Fig2: ICC staining MiTF in Hela cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig3: ICC staining MiTF in A431 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig4: ICC staining MiTF in NIH/3T3 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig5: Flow cytometric analysis of SW480 cells with MiTF antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.| Positive Control | A431, NIH/3T3, Hela, PC-12. |
|---|---|
| Application Notes | WB:1:1,000-1:2,000 FC:1:50-1:100 ICC:1:100-1:500 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
