Application
Fig1: Western blot analysis of Myosin heavy chain on human skeletal muscle lysates using anti-Myosin heavy chain antibody at 1/1,000 dilution.Application
Fig2: Immunohistochemical analysis of paraffin-embedded mouse smooth muscle tissue using anti-Myosin heavy chain antibody. Counter stained with hematoxylin.Application
Fig3: Immunohistochemical analysis of paraffin-embedded mouse heart tissue using anti-Myosin heavy chain antibody. Counter stained with hematoxylin.| Product Name | Myosin heavy chain [JF097-7] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | cardiac muscle alpha isoform antibody MYH6 antibody MYH6_HUMAN antibody MYH7 antibody MYHC antibody MyHC-alpha antibody MyHC-beta antibody MYHCA antibody MYHCB antibody Myosin heavy chain 6 antibody Myosin heavy chain antibody Myosin heavy chain cardiac muscle alpha isoform antibody Myosin heavy chain cardiac muscle beta isoform antibody Myosin-6 antibody |
| Product description | Myosin is a highly conserved, ubiquitously expressed protein that interacts with Actin to generate the force for cellular movements. Conventional myosins are hexameric proteins consisting of two heavy chain subunits, a pair of non-phosphorylatable light chain subunits and a pair of phosphorylatable light chain subunits. Three general classes of myosin have been cloned: smooth muscle myosins, striated muscle myosins and non-muscle myosins. Contractile activity in smooth muscle is regulated by the calcium/calmodulin-dependent phosphorylation of myosin light chain by myosin light chain kinase. Myosin heavy chains are encoded by the MYH gene family and have Actin-activated ATPase activity which generates the motor function of myosin. Myosin heavy chains, which were initially isolated from a human fetal skeletal muscle, are the major determinant in the speed of contraction of skeletal muscle. Various isoforms of myosin heavy chain are differentially expressed depending on the functional activity of the muscle. |
| Immunogen | recombinant protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Recombinant rabbit |
| Tested Applications | WBIHC |
| WB:1:1,000-1:2,000 IHC:1:50-1:200 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Alternative Names | cardiac muscle alpha isoform antibody MYH6 antibody MYH6_HUMAN antibody MYH7 antibody MYHC antibody MyHC-alpha antibody MyHC-beta antibody MYHCA antibody MYHCB antibody Myosin heavy chain 6 antibody Myosin heavy chain antibody Myosin heavy chain cardiac muscle alpha isoform antibody Myosin heavy chain cardiac muscle beta isoform antibody Myosin-6 antibody |
|---|---|
| Molecular Weight(MW) | 224 kDa |
| Cellular Localization | Cytoplasm. |
Application
Fig1: Western blot analysis of Myosin heavy chain on human skeletal muscle lysates using anti-Myosin heavy chain antibody at 1/1,000 dilution.
Application
Fig2: Immunohistochemical analysis of paraffin-embedded mouse smooth muscle tissue using anti-Myosin heavy chain antibody. Counter stained with hematoxylin.
Application
Fig3: Immunohistochemical analysis of paraffin-embedded mouse heart tissue using anti-Myosin heavy chain antibody. Counter stained with hematoxylin.| Positive Control | Human skeletal muscle tissue, mouse smooth muscle tissue, mouse heart tissue. |
|---|---|
| Application Notes | WB:1:1,000-1:2,000 IHC:1:50-1:200 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
