Application
Fig1: Western blot analysis of TRX1 on different lysates using anti-TRX1 antibody at 1/1,000 dilution. Positive control: Lane 1: Hela Lane 2: 293TApplication
Fig2: Flow cytometric analysis of Hela cells with TRX1 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.| Product Name | Thioredoxin [JF101-8] |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Thioredoxin (Trx) is a redox protein that is found in several species, such as bacteria, plants and mammals, and contains a conserved active site, consisting of Trp-Cys-Gly-Pro-Cys. Trx has several biological functions. It acts as a hydrogen donor for ribonucleotide reductase, which is critical for DNA synthesis, and modulates the DNA-binding activity of several transcription factors, including NFkB, AP-1, p53, TFIIIC and glucocorticoid receptor. Trx also stimulates cell growth, is an inhibitor of apoptosis and plays a role in the protection against oxidative stress. Drugs that inhibit Trx have antitumor activity, suggesting that Trx is involved in a variety of human diseases, including cancer. TrxR is a ubiquitously expressed flavoprotein that catalyzes the NADPH-dependent reduction of Trx as well as several other oxidized cellular components. |
| Immunogen | recombinant protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Recombinant rabbit |
| Tested Applications | WB,FC |
| WB:1:1,000-1:5,000 FC:1:50-1:100 |
|
| Species Reactivity | Human,Mouse,Rat |
| Concentration | 1mg/ml |
| Purification | Unpurified |
| Alternative Names | ADF antibody ATL derived factor antibody ATL-derived factor antibody DKFZp686B1993 antibody MGC61975 antibody SASP antibody Surface associated sulphydryl protein antibody Surface-associated sulphydryl protein antibody testicular tissue protein Li 199 antibody THIO_HUMAN antibody Thioredoxin antibody thioredoxin delta 3 antibody TRDX antibody TRX 1 antibody Trx antibody TRX1 antibody TXN antibody TXN delta 3 antibody TXN protein antibody zgc:92903 antibody |
|---|---|
| Molecular Weight(MW) | 12 kDa |
| Cellular Localization | Nucleus, Cytoplasm, Secreted. |
Application
Fig1: Western blot analysis of TRX1 on different lysates using anti-TRX1 antibody at 1/1,000 dilution. Positive control: Lane 1: Hela Lane 2: 293T
Application
Fig2: Flow cytometric analysis of Hela cells with TRX1 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.| Positive Control | 293T, Hela. |
|---|---|
| Application Notes | WB:1:1,000-1:5,000 FC:1:50-1:100 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
