Application
Fig1: Western blot analysis of ARF6 on human liver lysates using anti-ARF6 antibody at 1/1,000 dilution.Application
Fig2: ICC staining ARF6 in Hela cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig3: ICC staining ARF6 in MCF-7 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig4: ICC staining ARF6 in HepG2 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.| Product Name | ARF6 [JF101-2] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | ADP ribosylation factor 6 antibody ADP ribosylation factor protein 6 antibody ADP-ribosylation factor 6 antibody ARF6 antibody ARF6_HUMAN antibody DKFZp564M0264 antibody Small GTP binding protein antibody Small GTPase antibody |
| Product description | The ADP-ribosylation factor (ARF) protein family are structurally and functionally conserved members of the Ras superfamily of regulatory GTP-binding proteins. ARFs influence vesicle trafficking and signal transduction in eukaryotic cells. ARF-dependent regulatory mechanisms include the coordination of spectrin interactions with golgi membranes and the association of Actin to the Golgi via rho family-dependent G-protein localization (Rac, CDC42) and WASP/Arp2/3 complexes. Additionally, ARFs play a central role in maintenance of organelle integrity, assembly of coat proteins and activation of phospholipase D. The ARF proteins are categorized as class I (ARF1, ARF2 and ARF3), class II (ARF4 and ARF5) and class III (ARF6); members of each class share a common gene organization. The human ARF6 gene contains five exons and four introns, and encodes a 175 amino acid protein. |
| Immunogen | recombinant protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Recombinant rabbit |
| Tested Applications | WBICC/IF |
| WB:1:1,000-1:2,000 ICC:1:100-1:500 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Alternative Names | ADP ribosylation factor 6 antibody ADP ribosylation factor protein 6 antibody ADP-ribosylation factor 6 antibody ARF6 antibody ARF6_HUMAN antibody DKFZp564M0264 antibody Small GTP binding protein antibody Small GTPase antibody |
|---|---|
| Molecular Weight(MW) | 20 kDa |
| Cellular Localization | Golgi apparatus, Cell membrane, Endosome membrane, Cell projection, Midbody, Cytoplasm, Cleavage furrow. |
Application
Fig1: Western blot analysis of ARF6 on human liver lysates using anti-ARF6 antibody at 1/1,000 dilution.
Application
Fig2: ICC staining ARF6 in Hela cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig3: ICC staining ARF6 in MCF-7 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig4: ICC staining ARF6 in HepG2 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.| Positive Control | MCF-7, Hela, HepG2, human liver tissue. |
|---|---|
| Application Notes | WB:1:1,000-1:2,000 ICC:1:100-1:500 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
